摘要
目的探讨乳腺癌组织的长链非编码RNA LINC01296水平及临床意义。方法采用实时定量PCR检测本院2015年1月至2018年12月手术切除的166对癌组织和癌旁组织的LINC01296水平,受试者工作特征曲线评价组织LINC01296水平诊断乳腺癌的效能,分析LINC01296水平与乳腺癌临床病理特征的关系。Kaplan-Meier Plotter数据库分析LINC01296水平与乳腺癌预后指标的关系。结果 166例乳腺癌组织的LINC01296水平为2.810±1.219,高于对应癌旁组织的1.070±0.721(P<0.05);组织LINC01296诊断乳腺癌的曲线下面积为0.886,最佳截断点为2.340时约登指数最大(0.5904),灵敏度和特异度分别为63.86%和95.18%。LINC01296表达与肿瘤大小、淋巴结转移、TNM分期、HER-2表达和Nottingham预后指数有关(P<0.05),而与年龄、绝经、组织学分级、ER和PR表达均无关(P>0.05)。LINC01296表达与乳腺癌的总生存期、无病生存期和无远处转移生存期有关,而与进展后生存期无关。结论 LINC01296在乳腺癌中高表达且参与了肿瘤的发生发展,高水平LINC01296与不良预后的关系密切,可作为乳腺癌预后预测和治疗的潜在靶点。
Objective To investigate the level of long non-coding RNA LINC01296 in breast cancer tissues and its clinical significance. Methods Real-time quantitative PCR was used to detect the LINC01296 level in 166 pairs of cancerous and paracancerous tissues surgically removed from January 2015 to December 2018 in our hospital. Receiver operator characteristics curve was used to evaluate the efficacy of LINC01296 in the diagnosis of breast cancer, and the relationship between LINC01296 and the clinicopathological features of breast cancer was analyzed. Kaplan-Meier plotter database was used to analyze the relationship between LINC01296 level and prognosis of breast cancer. Results In 166 cases of breast cancer, the level of LINC01296 was 2.810±1.219, higher than 1.070±0.721 of adjacent tissues(P<0.05). The area under the curve of LINC01296 was 0.886, and the best cutoff point was 2.340, when the yoden index was the largest(0.5904), with the sensitivity and specificity of 63.86% and 95.18%, respectively. The expression of LINC01296 was related to tumor size, lymph node metastasis, TNM stage, HER-2 expression and Nottingham prognostic index(P<0.05), but not to age, menopause, histological grade, ER and PR expression(P>0.05). Expression of LINC01296 in breast cancer was related to overall survival, relapse-free survival and distant metastasis-free survival, but not progression-free survival. Conclusion LINC01296 is highly expressed in breast cancer and participates in the occurrence and development of breast cancer. High level LINC01296 is closely related to poor prognosis, which can be used as a potential target for prognosis prediction and treatment of breast cancer.
作者
李信
易军
贠军
徐昕
李一可
魏洪亮
LI Xin;YI Jun;YUN Jun;XU Xin;LI Yike;WEI Hongliang(Department of Thyroid and Breast Surgery,Xijing Hospital of Air Force Military Medical University,Xi an 710032,China)
出处
《临床肿瘤学杂志》
CAS
北大核心
2019年第11期1032-1035,共4页
Chinese Clinical Oncology
