摘要
目的探讨脐血采集和处理过程中微生物的污染情况,为降低脐血污染率提供参考。方法收集2014年8月—2018年7月上海市脐带血造血干细胞库保存的脐血样本25202份,同时进行需氧菌(含兼性厌氧菌)和厌氧菌培养,对培养出的细菌进行鉴定,并对检测结果进行分析。结果25202份脐血样本中分离出细菌458株,污染率为1.82%,其中需氧菌210株(45.85%)、厌氧菌248株(54.15%)。458株细菌中,剖宫产脐血样本污染菌23株(5.02%)、顺产脐血样本污染菌435株(94.98%);大肠埃希菌142株(31.00%)、脆弱拟杆菌51株(11.14%)、多形拟杆菌36株(7.86%)、粪肠球菌22株(4.80%)、痤疮丙酸杆菌18株(3.93%);厌氧菌中,顺产脐血样本较剖宫产脐血样本多检出了双歧杆菌属、消化链球菌属和梭菌属菌株。结论脐血污染细菌中,厌氧菌多于需氧菌;相对于剖宫产脐血样本,顺产脐血样本污染率较高,污染菌中厌氧菌种类较多。
Objective To investigate the microbial contamination during cord blood collection and treatment,and to provide a reference for reducing cord blood microbial contamination rate.Mothods A total of 25202 cord blood samples were collected from Shanghai Cord Blood Hematopoietic Stem Cell Bank from August 2014 to July 2018,and the culturing of aerobes(including facultative anaerobes)and anaerobes were carried out,and the identification was performed.Results A total of 458 isolates were determined,the total contamination rate was 1.82%.Among them,210 isolates were aerobes(45.85%),and anaerobes(248 isolates)accounted for 54.15%.The proportion of cord blood samples from cesarean section was 5.02%(23 isolates).The proportion of cord blood samples from eutocia was 94.98%(435 isolates).There were 142 isolates of Escherichia coli(31.00%),51 isolates of Bacteroides fragilis(11.14%),36 isolates of Bacteroides thetaiotaomicron(7.86%),22 isolates of Enterococcus faecalis(4.80%)and 18 isolates of Propionibacterium acnes(3.93%).Among the anaerobes,Bifidobacterium,Peptostreptococcus and Clostridium had been found more in eutocia than those in cesarean section.Conclusions Anaerobes are more common than aerobes in cord blood.The contamination rate in eutocia is high,and the species of anaerobes in eutocia are more than those in cesarean section for cord blood microbial contamination.
作者
张敏敏
刘学杰
金魏名
王敬华
陈蓉
王庆忠
徐蓉
葛平
周靖
LIU Xuejie;JIN Weiming;WANG Jinghua;CHEN Rong;WANG Qingzhong;XU Rong;GE Ping;ZHOU Jing(Shanghai Center for Clinical Laboratory,Shanghai 200126,China;Shanghai Cord Blood Bank,Shanghai 200051,China)
出处
《检验医学》
CAS
2019年第12期1088-1090,共3页
Laboratory Medicine
基金
上海市卫生和计划生育委员会面上项目（20134010）
上海市卫生和计划生育委员会面上项目（201840005）
河南省科技厅横向联合项目（181200211900）
关键词
脐血
污染
需氧菌
厌氧菌
Cord blood
Contamination
Aerobe
Anaerobe
