摘要
【目的】探究杨柳田头菇YAAS711菌株的单孢菌株及其自交个体的胞外酶活性变化规律,为其遗传育种提供理论依据。【方法】以杨柳田头菇YAAS711的18个单核体与22个自交双核体为试验材料,分别采用3,5-二硝基水杨酸(DNS)法、福林法、愈创木酚法等测定羧甲基纤维素酶、滤纸纤维素酶、β-葡萄糖苷酶、半纤维素酶、淀粉酶、蛋白酶、漆酶、多酚氧化酶、过氧化物酶9种胞外酶活性,并对这9种胞外酶与子代生长速度、极性和交配因子进行相关性分析。【结果】单核体和双核体绝大多数都具有较高的淀粉酶、滤纸纤维素酶、半纤维素酶、β-葡萄糖苷酶、羧甲基纤维素酶及蛋白酶活性,过氧化物酶、多酚氧化酶及漆酶活性却较低。9种胞外酶平均活力与单核体和双核体的菌丝生长速度均存在一定程度的相关性,除部分类型Ⅱ单核体外,菌丝生长速度较快的单核体具有较高的胞外酶活性;反之,胞外酶活性较低。相比单核体菌丝生长速度“快-快”的组合,单核体菌丝生长速度“快-慢”的组合除半纤维素酶、蛋白酶和多酚氧化酶外,其他胞外酶活性都较高。具有类型Ⅱ单核体参与形成的双合体胞外酶活性却各不相同且无规律。多酚氧化酶对单核体菌丝生长速度的间接影响大于漆酶,但漆酶对单核体菌丝生长速度的综合作用最大;胞外酶之间的协同作用对双核体菌丝生长速度影响较小,其中漆酶对其菌丝生长速度直接作用最大。多重比较结果显示,在显著水平为0.05上,极性对胞外酶平均活力的影响大于交配因子。【结论】单核体菌丝生长速度与本极性异常和杨柳田头菇YAAS711子代个体胞外酶活性差异存在显著相关性,除部分类型Ⅱ单核体和双核体外,类型Ⅰ菌株胞外酶活性具有相同的规律。单核体菌丝生长速度、极性、胞外酶三者之间具有一定的相关性,但交配因子与�
【Objective】The objective of this study was to explore the changes in activities of extracellular enzymes(EEA)of single-spore isolations and its self-crossing individuals from Agrocybe salicacola YAAS711 and to provide evidence for its genetic breeding.【Method】Eighteen single-spore monokaryons and 22 dikaryons from self-crossing progeny of A.salicacola YAAS711 were used as the test materials.The activities of CMCase,filter paper cellulase,β-glucanase,hemicellulase,amylase,protease,laccase,polyphenolase and peroxidase of all test strains were determined by 3,5-dinitrosalicylic acid(DNS),Folin methods and guaiacol method,and correlation analysis were carried out between the 9 EEs with mycelial growth rate,polarities and mating factors.【Results】The results of 9 EEs showed that most of the both monokaryons and dikaryons had higher activities of amylase,filter paper cellulase,hemicellulose,β-glucanase,CMCase and protease,but their activities of peroxidase,polyphenolase and laccase were low.The mycelial growth rate of monokaryons and dikaryons had a certain degree of correlation with the average activity of 9 EEAs.Compared with the dikaryons derived from the combination of“fast-fast”growth rate of monokaryons,the dikaryons derived from the combination of“fast-slow”growth rates of monokaryons had higher EEAs,except hemicellulase,protease and polyphenol oxidase activities.However,there were no rules to follow,when it came to the combination of dikaryons from the cross of type Ⅱ monokaryons.The indirect effect of polyphenolase on the mycelial growth rate of monokaryons was greater than that of laccase,but the combined effect of laccase on the mycelial growth rate of monokaryons was the largest.The synergistic effect among EEs had little effect on the mycelial growth rate of dikaryons,among them,laccase had the greatest direct effect on its mycelial growth rate.Multiple comparisons showed that the effect of polarity on EEs was greater than that of mating factors at a significant level of 0.05.【Conc
作者
周会明
赵永昌
柴红梅
张焱珍
ZHOU HuiMing;ZHAO YongChang;CHAI HongMei;ZHANG YanZhen(School of Biotechnology and Engineering,West Yunnan University,Lincang 677000,Yunnan;Institute of Biotechnology and Germplasmic Resource,Yunnan Academy of Agricultural Sciences,Kunming 650205)
出处
《中国农业科学》
CAS
CSCD
北大核心
2019年第20期3672-3684,共13页
Scientia Agricultura Sinica
基金
云南省教育厅科学研究基金(2018JS522)
国家食用菌产业技术体系(CARS-20)
关键词
杨柳田头菇
自交后代
胞外酶活性
单核体
双核体
Agrocybe salicacola
self-crossing F1 generations
extracellular enzyme activity
monokaryon
dikaryon
