摘要
利用HPD-100大孔树脂柱色谱、硅胶柱色谱、ODS柱色谱等分离纯化方法,从黄皮[Clausena lansium(Lour.)Skeels]中分离得到3个呋喃香豆素化合物,并综合运用波谱学分析方法对化合物进行结构测定。确定其结构分别为:8-[(2S,3S,6E)-2,3-epoxy-3,7-dimethyl-oct-6-enyloxy]psoralen(1)、8-(7',8'-epoxygeranyloxy)psoralen(2)和8-[(2E)-6-oxo-3,7-dimethyloct-2-enyloxy]psoralen(3)。其中,化合物1为新的呋喃香豆素。对化合物1~3进行肿瘤细胞毒活性测试,结果显示,化合物2对人大细胞肺癌细胞(H460)具有抑制活性(IC50=43.94μmol·L^-1);化合物3对人宫颈癌细胞(HeLa)具有抑制活性(IC50=33.76μmol·L^-1)。
One undescribed and two known furocoumarins were isolated from the stems of the Clausena lansium through a series of isolation and purification approaches including HPD-100 macroporous resin column,silica gel,reverse phase C18 and so on.Their structures were determined to be 8-[(2S,3S,6E)-2,3-epoxy-3,7-dimethyl-oct-6-enyloxy]psoralen(1),8-(7',8'-epoxygeranyloxy)psoralen(2)and 8-[(2E)-6-oxo-3,7-dimethyloct-2-enyloxy]psoralen(3)by spectroscopic methods.Compound 1 is a new furocoumarin.Compound 2 showed cytotoxicity to H460(IC50=43.94μmol·L^-1)and compound 3 showed cytotoxicity to HeLa(33.76μmol·L^-1)through the cytotoxic tests against five human cancer cell lines(H460,H7402,HCT-8,HeLa and MCF-7)for all compounds.
作者
孙兴妍
马洁
李创军
黄积武
王楠
沈苍颉
张东明
SUN Xing-yan;MA Jie;LI Chuang-jun;HUANG Ji-wu;WANG Nan;SHEN Cang-jie;ZHANG Dong-ming(State Key Laboratory of Bioactive Substance and Function of Natural Medicines,Institute of Materia Medica,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100050,China)
出处
《药学学报》
CAS
CSCD
北大核心
2019年第12期2282-2285,共4页
Acta Pharmaceutica Sinica
基金
北京市自然科学基金青年项目(7194299)
国家自然科学基金资助项目(NNSFC 81730093)
关键词
黄皮
化学成分
呋喃香豆素
细胞毒活性
Clausena lansium
chemical constituents
furocoumarin
cytotoxicity
