摘要
醛固酮是一种类固醇激素,通过作用于肾脏来维持机体的水钠平衡。原发性醛固酮增多症(简称原醛)是最常见的引起内分泌性高血压的疾病,其临床表现隐匿,具有较高的死亡率。肾素-醛固酮比值(ARR)、血/尿醛固酮的检测是最有效筛查和确诊原醛的方法。人体内醛固酮含量低且存有类似结构的干扰物质,因此对其准确定量具有一定的挑战性。醛固酮检测方法有放射免疫法、酶联免疫吸附法、化学发光法和质谱。每种方法具有各自的优缺点,且不同检测方法诊断原醛的ARR比值也不完全一致。因此本文对醛固酮检测方法及临床应用的研究进展进行综述。
Aldosterone is a steroid hormone that acts on the kidneys to maintain the balance of sodium and water.Primary hyperaldosteronism(PA)is the most common disease that causes secretory hypertension.PA is often unrecognized and is associated with a high death rate.The aldosterone/rein rate(ARR)and detection of serum or urinary aldosterone are the most effective method to screen and identify PA.In healthy individuals,aldosterone concentration is present in low quantities in serum and there are numerous compounds that can potentially interfere with analysis making aldosterone a technically challenging analyte quantifying method.At present,the method of aldosterone detection includes radioimmunoassay,enzyme-linked immunosorbent assay,chemiluminescence method and mass spectrometry.These methods have their own advantages and disadvantages,the ARR ratios of different detection methods for the diagnosis of PA are not completely consistent.In this paper,the detection methods and clinical applications of aldosterone are reviewed.
作者
钱婧雨
刘向祎
Qian Jingyu;Liu Xiangyi(Department o f Clinical Laboratory,Beijing Tongren Hospital,Capital Medical University,Beijing 100730,China)
出处
《中华检验医学杂志》
CAS
CSCD
北大核心
2019年第12期1072-1077,共6页
Chinese Journal of Laboratory Medicine
关键词
醛固酮
放射免疫测定
酶联免疫吸附测定
质谱分析法
Aldosterone
Radioimmunoassay
Enzyme-linked immunosorbent assay
Mass spectrometry
