摘要
目的探讨血管性血友病因子(von Willebrand Factor,vWF)对高脂饮食诱导的肥胖及内脏脂肪含量、组织炎症的影响。方法 16只8周大雄性WT小鼠随机分为普通饮食组(WTNC)、高脂饮食组(WT HFD),16只8周大vWF基因敲除小鼠随机分为普通饮食组(vWFKONC)、高脂饮食组(vWFKOHFD),一共四组,每组8只,普通饮食组给予普通饲料喂养,高脂饮食组给予60%高脂饲料喂养。12周后对比各组小鼠体重、附睾脂肪含量及附睾脂肪的病理形态和炎症表达水平。结果相较于普通饮食,高脂饮食可显著增加WT、vWF基因敲除小鼠的体重及附睾脂肪含量;而对比高脂饮食vWF基因敲除和高脂饮食WT小鼠,发现前者的体重、附睾脂肪含量均明显小于后者[体重:(30.44±0.60) g vs (43.74±0.78) g,附睾脂肪含量:(1.14±0.11) g vs (3.01±0.04) g,P值均<0.05],进一步对上述2组附睾脂肪组织进行HE病理分析,发现前者附睾脂肪中"王冠样结构"[1]的数量少于后者,且附睾脂肪RT-PCR分析显示前者MCP-1、TNF-α、IL-β和IL-6的表达均低于后者(P值均<0.05)。结论 vWF基因敲除可抑制高脂饮食小鼠的体重增长和附睾脂肪含量,同时可降低脂肪组织炎症水平。
Objective To explore the effects of von Willebrand Factor on diet-induced obesity(DIO),visceral fat accumulation and adipose tissue inflammation in mice.Method 16 8-week-old SPF WT male mice were randomly divided into two groups:the mice fed a high-fat diet(WT HFD) or a normal chow diet(WT NC).16 age-and sex-matched vWF-knockout mice were also randomly distributed into group fed a high-fat diet(vWF KO HFD) or a normal chow diet(vWF KO NC).There are 4 groups in total,8 in each.After 12 weeks’ of feeding,comparisons of body weight,epididymal white adipose tissue(eWAT) mass and adipose tissue inflammation were made among different groups.Results Compared with the mice fed a NC diet,both WT and vWF-knockout mice on HFD gained more bodyweight and eWAT mass.However,the bodyweight and eWAT mass in vWF-knockout mice on HFD were lighter than those in WT mice on the same diet [bodyweight(30.44±0.6) g vs(43.74±0.78) g,eWAT mass(1.14±0.11) g vs(3.01±0.04) g,P<0.05].HE analysis of eWAT showed less crown like structure in vWF-knockout mice than in WT mice on HFD.Meanwhile,the relative mRNA expression of MCP-1,TNF-a,IL-1β and IL-6 were significantly lower in vWF-knockout mice than in WT mice on HFD(P<0.05 for each cytokine).Conclusion Knockout of vWF prohibits mice on HFD from bodyweight gain,eWAT accumulation and adipose tissue inflammation.
作者
陆艳
杨娟
汪海东
Yang Juan;Wang Haidong(Department of Endocrinology and Metabolism,Huadong Hospital Affiliated to Fudan University,Shanghai,20040,P.R.China)
出处
《老年医学与保健》
CAS
2019年第6期778-782,共5页
Geriatrics & Health Care
基金
上海市自然科学基金(14ZR1413200)
上海市卫生计生系统重要薄弱学科建设规划—老年医学(2015ZB0501)
上海市老年医学临床重点实验室(13DZ2260700)
关键词
血管性血友病因子
基因敲除
高脂饮食
附睾脂肪含量
王冠样结构
脂肪组织炎症
von Willebrand Factor(vWF)
gene knockout
high-fat diet(HFD)
epididymal fat content
crown-like structure
adipose tissue inflammation
