摘要
为筛选有效的抗结肠小袋纤毛虫药物,本研究选取替硝唑、阿奇霉素等10种广谱抗细菌、真菌和原虫药物,体外检测了这些药物对小袋虫滋养体抑杀效果。参考这些药物对细菌、真菌或原虫的治疗剂量,设置高、中、低剂量来确定药物是否有效,然后用不同浓度的急性毒性试验来筛选有效药物的半数致死浓度(LC50)和最小杀虫浓度(MIC)。结果显示,10种药物中有6种药物对小袋虫有抑杀作用,分别是替硝唑、阿奇霉素、妥曲珠利、四环素、青蒿素和常山酮,药物作用6 h的LC50(LC50,6h)分别是5.0606μg/mL、1.0647 mg/mL、0.5979 mg/mL、1.5272 mg/m L、1.3079 mg/mL、0.5573 mg/mL,小袋虫滋养体在这6种药物的作用下,其数量及运动活性均有明显的减少和抑制,其中替硝唑和阿奇霉素的效果最明显,均在加药后6 h内观察到小袋虫滋养体的数量明显减少,滋养体运动活性较对照组虫体明显缓慢,然后崩解。本研究首次发现阿奇霉素、妥曲珠利、青蒿素和常山酮对小袋虫具有抑杀效果,为临床上用药物来控制动物和人的小袋虫病提供更多选择。
In order to screen effective anti-Balantioides coli drugs,ten drugs were selected to examine their inhibitory efficacy against trophozoites of B.coli.These ten drugs including Tinidazole,Azithromycin,Toltrazuril,Tetracycline,Artemisinin,Halofuginone,Amphotericin B,Paromomycin,Gentamicin and Anisomycin that have broad-spectrum anti-bacterial,anti-fungi or anti-protozoal effect.Refer to the therapeutic dose of these drugs,we set up high,medium and low doses in the preliminary experiments to determine whether the drug is effective.For those effective drugs,acute toxicity tests using different concentration were performed to screen the median lethal concentrations(LC50)and the minimum insecticidal concentrations(MIC).The results showed that 6 of the 10 drugs had inhibitory efficacy on B.coli trophozoites,namely Tinidazole,Azithromycin,Toltrazuril,Tetracycline,Artemisinin and Halofuginone.The LC50,6 hvalues of these six effective drugs were 5.0606μg/m L,1.0647 mg/m L,0.5979 mg/m L,1.522 mg/m L,1.3079 mg/m L and 0.5573 mg/m L,respectively.Under the drugs,the quantities of trophozoites were significantly decreased,and motion activity of trophozoites was distinctly inhibited.The inhibitory efficacy of Tinidazole and Azithromycin against trophozoites was the strongest among the six drugs.6 hours after Tinidazole and Azithromycin administration,the number of trophozoites was significantly reduced,and the motion activity was slower than that of the control group,and then disintegrated.This study reported that Azithromycin,Toltrazuril,Artemisinin and Halofuginone have an inhibitory effect on B.coli reports for the first time,and will provide more options for clinical application of drugs to control balantidiosis in humans and animals.
作者
孙晨阳
闫文朝
何凯
王天奇
SUN Chen-yang;YAN Wen-chao;HE Kai;WANG Tian-qi(College of Animal Science and Technology,Henan University of Science and Technology,Luoyang 471023,China)
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2019年第11期1120-1125,共6页
Chinese Journal of Preventive Veterinary Medicine
基金
国家自然科学基金项目(31772733)
