摘要
[目的]为了掌握肉牛口蹄疫免疫抗体检测结果、口蹄疫非结构蛋白3ABC抗体检测结果、口蹄疫O-P液RT-PCR核酸检测结果三者的相关性,[方法]张掖市动物疫病预防控制中心对甘州区28头肉牛开展相关检测。[结果]检测结果表明:O型口蹄疫免疫抗体合格率28份,合格率100%,3ABC抗体阳性27份,阳性率96.4%;对3ABC抗体阳性的27头牛分2次进行实时荧光RT-PCR核酸检测,被检的27头牛均为阴性。
[Objective]In order to master the correlation among the detection results of foot and mouth disease(FMD)immune antibody,FMD nonstructural protein 3 ABC antibody and FMD O-P solution RT-PCR nucleic acid,[Method]Zhangye animal disease prevention and control center carried out the relevant detection on 28 beef cattle in Ganzhou district.[Result]The results showed that the qualified rate of O-FMD antibody was 28,the qualified rate was 100%,the positive rate of 3 ABC antibody was 27,the positive rate was 96.4%;27 cattle with 3 ABC antibody positive were detected by real-time RT-PCR twice,and all 27 cattle were negative.
作者
朵玉明
何彦春
DUO Yu-ming;HE Yan-chun(The Technical Service Center of Animal Husbandry and Veterinary,Sunan County,Sunan,Gansu 734000;Zhangye Animal Disease Prevention and Control Center,Zhangye,Gansu 734000)
出处
《中国牛业科学》
2019年第6期29-30,共2页
China Cattle Science
关键词
肉牛
口蹄疫
免疫抗体
3ABC抗体
RT-PCR核酸检测
beef cattle
foot and mouth disease
immune antibody
3ABC antibody
RT-PCR nucleic acid detection
