摘要
目的:研究铁皮石斛多糖不同分级组分对小鼠免疫调节及肠道健康的影响。方法:利用梯度醇沉法对铁皮石斛精多糖进行相对分子质量的分级处理。以环磷酰胺诱导免疫抑制小鼠模型,通过灌胃铁皮石斛多糖不同分级组分,比较灌胃前、后小鼠胸腺指数、血清和脾脏细胞因子分泌量的变化,探究铁皮石斛多糖不同分级组分对小鼠的免疫调节作用。同时测定模型组与多糖分级组的粪便含水量、pH值和短链脂肪酸含量水平,研究铁皮石斛多糖分级组分对肠道健康的影响。结果:与模型组相比,DOP组和F50S组均能显著提高小鼠胸腺指数,促进IFN-γ、TNF-α、IL-6的分泌,抑制IgE的分泌,在一定程度上引导免疫类型向Th1型偏移,提高小鼠的免疫功能;并能降低粪便pH,提高粪便含水量和SCFAs含量水平,从而促进肠道健康。结论:铁皮石斛多糖不同分级组分均能有效增强免疫低下小鼠的免疫调节作用,并改善其肠道健康,DOP比各分级组分的作用效果好;各分级组分中,相对分子质量最小的F50S作用效果较其它组分更为明显。
Objective:To study the immunomodulation and colon health effect of polysaccharide fractions from Dendrobium officinale in mice.Methods:The polysaccharides isolated by alcohol precipitation of Dendrobium officinale and have different molecular weights.The immunosuppressed mouse model was established by intraperitoneal injection of cyclophosphamide.To evaluate the immunomodulation effect of polysaccharide fractions from Dendrobium officinale,the changes of thymus index,serum and spleen cytokine secretion were determined before and after gavage of the polysaccharides.The levels of water content,pH value and short-chain fatty acid content of feces in cyclophosphamide model group and polysaccharide fractions groups were also measured to evaluate the effect of polysaccharide fractions on colon health.Results:Compared with the cyclophosphamide model group,DOP group and F50S group can significantly improve the thymus index of mice;promote the secretion of IFN-γ,TNF-α,IL-6;inhibit the secretion of IgE;regulate the balance of Th1/Th2 cell responses shift to Th1 cell responses then enhance the immune function of mice.In addition,they can reduce the pH of feces,increase fecal water content and SCFAs levels which could promote colon health.Conclusion:polysaccharide fractions from Dendrobium officinale can effectively enhance the immune regulation of immunosuppressed mice and improve their colon health,however,DOP is more effective than each polysaccharide fraction.In addition,the effect of F50S fraction with the smallest Mw is better than other fractions.
作者
张珊珊
童微
胡婕伦
黄晓君
殷军艺
聂少平
Zhang Shanshan;Tong Wei;Hu Jielun;Huang Xiaojun;Yin Junyi;Nie Shaoping(State Key Laboratory of Food Science and Technology,Nanchang University,Nanchang 330047)
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2019年第12期14-21,共8页
Journal of Chinese Institute Of Food Science and Technology
基金
江西省主要学科学术带头人资助计划(20162BCB22008)
江西省青年科学基金项目(重点项目)(20171ACB21013)
江西省优势科技创新团队建设计划项目(20165BCB19001)
《中国-加拿大科学技术和创新行动计划(2016-2018))》
农业与食品领域合作框架合作项目(2017ZJGH0102001)
关键词
铁皮石斛多糖
梯度醇沉分级
免疫调节
肠道健康
Dendrobium officinale polysaccharides
gradient alcohol precipitation
immune regulation
colon health
