摘要
建立同时测定11种游离氨基酸与11种核苷类成分含量的超快速液相色谱-四极杆离子阱质谱联用方法,并分析明党参原植物和组织培养样品中游离氨基酸及核苷类含量。采用Waters XBridge Amide(2.1 mm × 100 mm,3.5 μm)色谱柱,柱温30 ℃,0.2%甲酸-0.2%甲酸乙腈溶液以0.6 mL·min^-1流速梯度洗脱,以多反庶监测模式进行正离子检测,结合主成分分析法和逼近理想点排序法探讨不同样品品质差异。结果表明,精密度、重复性、稳定性和回收率考察结果相对标准偏差均小于5%,加样回收率在98.62%~100.64%之间,被测成分线性关系良好,表明该检测方法准确、可行;明党参样品中游离氨基酸与核苷类成分差异较大,柄悬浮细胞测得游离氨基酸总含量(3682.01μg·g^-1)与核苷总含量(6241.90μg·g^-1)均为最高;分别以氨基酸和核苷为指标,主成分分析最高评分分属原植物根和柄悬浮细胞,逼近理想点排序表明柄悬浮细胞综合品质最优。本实验所建方法可用于明党参中22种游离氨基酸与核苷含量的同时快速测定,品质评价结果提示明党参根、叶、柄悬浮细胞和再生叶具开发利用价值,可为合理利用明党参资源提供参考。
UFLC-Qtrap-MS/MS method was established to simultaneously determine 11 kinds of free amino acids and 11 kinds of nucleosides in Changium smymioides Wollf and its in vitro cultures.Waters XBridge Amide column(2.1 mm × 100 mm,3.5 μm)was used for chromatographic separation at 30 Tl.Tlie flow rate was 0.6 mL·min^-1 with a mobile phase of 0.2% formic acid-0.2% formic acid in acetonitrile in gradient elution mode.The compounds were detected by positive ion multiple reaction monitoring mode.The results were analyzed by principal component analysis(PC A)and technique for order preference by similarity to an ideal solution(TOPSIS).The results showed that,relative standard deviations of precision,repeatability y stability and recovery tests were all less than 5%.The average recovery values were within 98.62%~100.64%.The linear relationship of measured components was good,which showed that the method was accurate and feasible.The contents of free amino acids and nuoleosides in different samples varied grealiy.The suspension cells from petiole contained the highest content of total free amino acids(3682.01μg·g^-1)and total nucleosides(6241.90μg·g^-1).The highest evaluation index of amino acids was in cultivated C.smymioides Wollf root while that of nucleosides was in suspension cells cultured from petiole.TOPSIS evaluation showed that the quality of petiole suspension cell was the best.The method established was suitable for simultaneous and rapid determination of 22 kinds of free amino acids and nucleosides in C.smymioides Wollf.The quality evaluation pointed out llie utility value of tlie root,leaf,petiole suspension cell and regenerated leaf of C.smymioides Wollf.The study provided some options for rational utilization of C.smyrnioides Wollf resource.
作者
蔡静
张园娇
胡鹏
陈建伟
李祥
CAI Jing;ZHANG Yuan-jiao;HU Peng;CHEN Jian-wei;LI Xiang(Changzhou TCM Hospital,Changzhou 213003,China;College of Pharmacy,Nanjing University of Chinese Medicine,Nanjing 210023,China)
出处
《食品工业科技》
CAS
北大核心
2019年第23期1-9,16,共10页
Science and Technology of Food Industry
基金
国家公益性行业科研专项(201407002)
江苏高校优势学科建设工程资助项目(ysxk-2010)
高等学校博士学科点专项科研基金(200803150009)。
关键词
明党参
氨基酸
核苷
含量测定
品质评价
Changium smyrnioides Wollf
amino acid
nucleoside
content determination
quality evaluation
