摘要
背景:目前有关复方脑肽节苷脂的研究大多集中在临床一些颅脑损伤和新生儿缺血缺氧性脑病的疗效观察和分析,但其参与神经功能恢复的分子机制少有报道。目的:探讨复方脑肽节苷脂在宫内缺氧新生鼠脑损伤中的保护作用及机制。方法:15只昆明小鼠随机分为对照组、缺氧组和治疗组。于孕14 d开始,缺氧组和治疗组孕鼠置于体积分数10%氧培养箱中,制作胎鼠宫内缺氧模型。孕鼠分娩后,新生鼠经腹腔分别注入复方脑肽节苷脂和PBS,治疗后观察各组小鼠发育情况;对照组不缺氧,不治疗。用免疫荧光和Western blot检测脑胶质纤维酸性蛋白、神经蛋白聚糖、突触分化诱导基因1蛋白和胶原蛋白Ⅳ的表达;小鼠跳台实验测试动物记忆功能。实验方案经漯河医学高等专科学校动物实验伦理委员会批准。结果与结论:①与对照组相比,缺氧组胶质纤维酸性蛋白和神经蛋白聚糖阳性细胞表达显著增加,而突触分化诱导基因1蛋白和胶原蛋白Ⅳ的表达显著减少;②治疗组突触分化诱导基因1蛋白和胶原蛋白Ⅳ表达均较缺氧组显著增加,而胶质纤维酸性蛋白和神经蛋白聚糖的表达较缺氧组显著减少;③与对照组相比,缺氧组大鼠学习记忆能力明显下降,经复方脑肽节苷脂注射液治疗后学习记忆能力明显改善;④结果说明,复方脑肽节苷脂可减轻缺氧后脑损伤,改善学习记忆能力,具体机制可能与抑制星形胶质细胞活化、下调神经蛋白聚糖和上调胶原蛋白Ⅳ表达,减轻脑血管损伤,促进突触的重建有关。
BACKGROUND:The most current application of compound porcine cerebroside and ganglioside injection(CPCGI)focuses on the clinical therapeutic efficacy in some craniocerebral injuries and neonatal hypoxic ischemic encephalopathy,but the molecular mechanism of CPCGI involved in the recovery of nerve function is rarely reported.OBJECTIVE:To study the protective effect and mechanism of CPCGI in a neonatal mouse model of intrauterine hypoxia-induced brain injury.METHODS:Fifteen Kunming mice were randomly divided into control group,hypoxia group and treatment group.At the 14th day after pregnancy,mice in hypoxia group and treatment group were put into a 10%oxygen incubator to make the model of intrauterine hypoxia.After delivery,neonatal mice were intraperitoneally injected with CPCGI and PBS respectively,and mouse development in each group was observed after treatment.Mice in the control group were not hypoxic and treated.The expression of glial fibrillary acidic protein,Neurocan,SynDIG1 and collagen IV were observed by immunofluorescence staining and western blot assay.A step-down test was used to test animal memory function.The study protocol was approved by the Animal Ethic Committee of Luohe Medical College in China.RESULTS AND CONCLUSION:Compared with the control group,the expressions of glial fibrillary acidic protein and Neurocan in the hypoxia group were significantly increased,while the expressions of SynDIG1 and collagen IV were significantly decreased.The expression of SynDIG1 and collagen IV in the treatment group was significantly increased than that in the hypoxia group,while the expression of glial fibrillary acidic protein and Neuroncan was significantly decreased than that in the hypoxia group.The learning and memory ability of mice in the hypoxia group was significantly decreased compared with the control group,but was significantly improved after CPCGI treatment.These results suggest that CPCGI can alleviate brain injury and improve learning and memory ability after hypoxic ischemic encephalopathy.The
作者
张福华
范文娟
华新宇
陈旭东
Zhang Fuhua;Fan Wenjuan;Hua Xinyu;Chen Xudong(Department of Traditional Chinese Medicine,Luohe Medical College,Luohe 462002,Henan Province,China;Laboratory for Molecular Medicine,Luohe Medical College,Luohe 462002,Henan Province,China;Department of Histology and Embryology,Luohe Medical College,Luohe 462002,Henan Province,China)
出处
《中国组织工程研究》
CAS
北大核心
2020年第11期1689-1694,共6页
Chinese Journal of Tissue Engineering Research
基金
河南省科技厅科技攻关项目(182102310083)，项目负责人：陈旭东~~
关键词
复方脑肽节苷脂注射液
宫内缺氧
胶原蛋白Ⅳ
脑损伤
河南省科技攻关项目
compound porcine cerebroside and ganglioside injection
intrauterine hypoxia
collagen IV
brain injury
Scientific and Technological Breakthrough Foundation of Henan Province
