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一例猪繁殖与呼吸综合征病的诊断 被引量:1

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摘要 河南某地区一猪场肥猪群出现严重呼吸道症状,母猪出现流产现象,病理剖检显示猪肺门淋巴结肿大,猪肺脏呈现间质性肺炎病变,边缘出现实变,临床症状显示疑似猪繁殖与呼吸综合征病(PRRS)阳性。为了确诊,从肺脏病变部位采集病料,提取RNA进行RT-PCR扩增,最后进行序列的遗传进化分析,结果显示扩增出猪繁殖与呼吸综合征病毒ORF5基因(ZMD),进化分析显示扩增出的序列与NADC30和NADC30-like序列位于同一分支,序列比对显示ZMD与欧洲型LV的核苷酸同源性为61.4%;和美洲型VR-2332毒株的同源性为85.1%;与经典疫苗株CH-1a核苷酸同源性为87.1%;和致病性很高的毒株JXA1核苷酸同源性为85.6%;与NADC30毒株核苷酸同源性最高,为95.9%。这些试验结果表明引起猪只发病的病原为PRRSV,毒株类型为NADC30-like毒株。
出处 《中国猪业》 2019年第8期79-81,共3页 China Swine Industry
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  • 1Bej A K,Mahbubani M H,Miller R,DiCesare J L,Haff L,Atlas R M.1990.Multiplex PCR amplification and immobilized capture probes for detection of bacterial pathogens and indicators in water.Molecular and Cellular Probes,4,353-365. 被引量:1
  • 2Belák S.2005.The molecular diagnosis of porcine viral diseases:a review.Acta Veterinaria Hungarica,53,113-124. 被引量:1
  • 3Belák S,Thorén P.2001.Molecular diagnosis of animal diseases:some experiences over the past decade.Expert Review of Molecular Diagnostics,1,434-443. 被引量:1
  • 4Collins J E,Benfield D A,Christianson W T,Harris L,Hennings J C,Shaw D P,Goyal S M,McCullough S,Morrison R B,Joo H S,et al.1992.Isolation of swine infertility and respiratory syndrome virus(isolate ATCC VR-2332)in North America and experimental reproduction of the disease in gnotobiotic pigs.dournal of Veterinary Diagnostic Investigation,4,117-126. 被引量:1
  • 5Edward M C,Gibbs R A.1994.Multiplex PCR:advantages,development and applications.PCR Methods and Application,3,65-75. 被引量:1
  • 6Elnifro E M,Ashshi A M,Cooper R J,Klapper P E.2000.Multiplex PCR:optimization and application in diagnostic virology.Clinical Microbiology Reviews,13,559-570. 被引量:1
  • 7Goyal S M.1993.Porcine reproductive and respiratory syndrome.Journal of Veterinary Diagnostic Investigation,5,656-664. 被引量:1
  • 8Henegariu O,Heerema N A,Dlouhy S R,Vance G H,Vogt P H.1997.Multiplex parameters and step-by-step protocol.Biotechniques,23,504-511. 被引量:1
  • 9Nidaira M,Taira K,Okano S,Shinzato T,Morikawa T,Tokumine M,Asato Y,Tada Y,Miyagi K,Matsuda S,et al.2009.Survey of Japanese encephalitis virus in pigs on Miyako,Ishigaki,Kume,and Yonaguni Islands in Okinawa,Japan.Japanese dournal of Infectious Diseases,62,220-224. 被引量:1
  • 10Ogawa H,Taira O,Hirai T,Takeuchi H,Nagao A,Ishikawa Y,Tuehiya K,Nunoya T,Ueda S.2009.Multiplex PCR and multiplex RT-PCR for inclusive detection of major swine DNA and RNA viruses in pigs with multiple infections.Journal of Virological Methods,160,210-214. 被引量:1

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