摘要
目的基于三七总皂苷(PNS)存在状态,明确溶液环境对其分离行为的影响。方法以成分透过率、溶液表面张力为指标,分析单因素乙醇、无机盐、表面活性剂和pH值对PNS存在状态的影响,进而采用Box-Behnken中心组合设计建立数学模型,考察乙醇浓度、氯化钠浓度、溶液pH值对三七皂苷R1(R1)和人参皂苷Rb1(Rb1)超滤分离的影响,分析因素交互作用,明确影响规律。结果乙醇可以降低皂苷分子间作用力,pH值促进皂苷离子化,增加临界胶束浓度,PNS超滤透过率增加;无机盐的盐析作用降低临界胶束浓度,PNS透过率降低;表面活性剂类型与PNS超滤分离行为相关;通过响应面分析成分超滤透过行为,Rb1对考察因素的敏感度高于R1。结论明确了溶液环境因素对PNS超滤分离的影响规律,可以动态调节成分状态,实现皂苷类成分的目的性分离。
Objective To clarify the effect of solution environment on ultrafiltration separation of Panax notoginseng total saponins(PNS)based on the molecular state.Methods In the experiment,the transmittance and surface tension were selected as indexes for analyzing the effect of ethanol,inorganic salts,surfactants,and pH on the molecular state of PNS.And then,ethanol,NaCl,and pH were selected as influencing factors to analyze the separation rule of notoginsenoside R1(R1)and ginsenoside Rb1(Rb1).Results The intermolecular interaction force of saponins was weakened by increasing the ethanol concentration;The pH value promoted saponin ionization,increased critical micelle concentration,and increased PNS ultrafiltration transmittance;The salting out effect of inorganic salt reduced the critical micelle concentration and PNS transmittance;The surfactant type was related to the ultrafiltration separation behavior of PNS.Rb1 was more sensitive to the factors than R1 by response surface methodology.Conclusion The effect of solution environmental factors on the ultrafiltration separation of PNS was clarified by the combination of single factor analysis and response surface methodology.And the saponins can be separated purposefully by dynamically adjusting the molecular state.
作者
李存玉
支兴蕾
牛学玉
戴凌婕
李红阳
彭国平
LI Cun-yu;ZHI Xing-lei;NIU Xue-yu;DAI Ling-jie;LI Hong-yang;PENG Guo-ping(College of Pharmacy,Nanjing University of Chinese Medicine,Nanjing 210023,China;Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization,Nanjing 210023,China)
出处
《中草药》
CAS
CSCD
北大核心
2019年第21期5246-5252,共7页
Chinese Traditional and Herbal Drugs
基金
国家自然科学基金资助项目(81603307)
江苏省高校自然科学基金(17KJB360010)
关键词
超滤
三七总皂苷
溶液环境
成分状态
三七皂苷R1
人参皂苷RB1
ultrafiltration
Panax notoginseng total saponins
solution environment
molecular state
notoginsenoside R1
ginsenoside Rb1
