摘要
目的解决玻片固定液干燥过快问题,改良固定液配方。方法为减缓固定液干燥速度,将5 ml甘油加入封片剂,保持湿度;并将原配方50%质量分数葡萄糖溶液5 ml改为3 ml,降低粘度。结果新的封片剂配方为阿拉伯胶12 g,水合氯醛晶体20 g,冰醋酸5 ml,甘油5 ml,50%质量分数葡萄糖溶液3 ml,蒸馏水30 ml。结论该封片剂干燥速度适中,适用于我国北方小型昆虫标本制备。
Objective To improve the formula of slide mounting medium, and to solve the problem that the slide mounting medium dries too fast. Methods In order to reduce the drying rate of the slide mounting medium, 5 ml glycerol was added to the medium to maintain the humidity;glucose was reduced from 5 ml(old formula) to 3 ml to reduce the viscosity.Results The new medium formula was as follows: acacia 12 g, chloral hydrate crystal 20 g, glacial acetic acid 5 ml,glycerol 5 ml, 50%w/w glucose syrup 3 ml, and distilled water 30 ml. Conclusion The drying rate of this slide mounting medium is in the moderate range, which is suitable for the preparation of small insect specimens in northern China.
作者
张娇
褚梦颖
王中印
刘广纯
ZHANG Jiao;CHU Meng-ying;WANG Zhong-yin;LIU Guang-chun(Liaoning Key Laboratory of Urban Integrated Pest Mangement and Ecological Security,College of Life Sciences and Bioengineering,Shenyang University,Shenyang 110044,Liaoning Province,China)
出处
《中国媒介生物学及控制杂志》
CAS
2019年第6期716-718,共3页
Chinese Journal of Vector Biology and Control
基金
国家自然科学基金(31372245)~~
关键词
蚤蝇
封片剂
玻片制作
Scuttle fly
Slide mounting medium
Slide preparation
