摘要
目的建立同时测定固龈液药渣中没食子酸、没食子酸甲酯和鞣花酸含量的方法,研究药渣干燥方法对其含量的影响。方法采用高效液相色谱(HPLC)切换波长法测定上述3种成分的含量,色谱柱为Agilent Zorbax SB-C18(250 mm×4.6 mm,5μm);流动相为甲醇(A)-0.05%磷酸水溶液(B),梯度洗脱程序,检测波长为272 nm(没食子酸、没食子酸甲酯),255 nm(鞣花酸),流速为1 ml/min,进样量3μl。采用晒干、鼓风干燥、真空干燥、微波真空干燥等方法,干燥固龈液药渣,并测定干燥药渣中3种成分的含量。结果没食子酸、没食子酸甲酯、鞣花酸的质量线性范围分别为1.280~4.608μg(r=0.9998)、0.560~2.016μg(r=0.9998)、0.1145~0.4122μg(r=0.9997);3种成分的平均回收率分别为99.97%、99.93%、100.20%,RSD分别为0.34%、2.30%、0.93%。在不同方法干燥的固龈液药渣中3种成分的含量均呈较大幅度变化。结论该法快速、简便、重现性好,可用于固龈液药渣中上述3种成分的含量测定。不同干燥方法对药渣中所含3种成分的含量有显著影响,相关结果可为后续研究提供参考。
Objective To establish a method for the simultaneous determination of gallic acid(GA),methyl gallate(MG)and ellagic acid(EA)in the residue of a gingival consolidation liquid(a mouth rinse preparation),and investigate the effect of different residue-drying methods on the GA,MG and EA content in the residues.Methods High performance liquid chromatography(HPLC)switching walvelength method was used to determine the GA,MG and EA contents.The column was Agilent Zorbax SB-C18(250 mm×4.6 mm,5μm).The mobile phase was methanol(A)-0.05%phosphoric acid aqueous solution(B)in a gradient elution.The detection wavelength was 272 nm for GA/MG and 255 nm for EA.The flow rate was 1 ml/min,and the injection volume was 3μl.Meanwhile,the residues were dried with the methods of sun drying,blast drying,vacuum drying and microwave vacuum drying,respectively and the GA,MG and EA contents in the residues were determined by the established HPLC method.Results The linear ranges for GA,MG and EA were 1.280-4.608(r=0.9998),0.560-2.016(r=0.9998),0.1145-0.4122μg(r=0.9997),respectively.The averagerecoveries for GA,MG and EA were 99.97%,99.93%and 100.20%,with the RSD of 0.34%,2.30%and 0.93%,respectively.The contents of GA,MG and EA varied in quite a large range in the residues dried by different methods.Conclusion The established method is fast,simple and practicable,which could be used for the determination of GA,MG and EA in the residue of a gingival consolidation liquid.The drying methods could significantly affect the contents of GA,MG and EA in the residues,and the related results provide a reference for future studies.
作者
宋倩倩
庞珊娇
李柯翺
田树革
SONG Qian-qian;PANG Shan-jiao;LI Ke-ao;TIAN Shu-ge(College of Chemistry and Chemical Engineering,Xinjiang Normal University,Urumqi 830054,China;College of Pharmacy,Xinjiang Medical University,Urumqi 830011,China;Xinjiang Qimu Medical Research Institute(Co.Ltd.),Urumqi 830011,China;College of TCM,Xinjiang Medical University,Urumqi 830011,China)
出处
《国际药学研究杂志》
CAS
北大核心
2019年第10期790-794,共5页
Journal of International Pharmaceutical Research
基金
新疆维吾尔自治区科技厅重大专项资助项目(2017A03005-2)
关键词
固龈液药渣
干燥方法
没食子酸
没食子酸甲酯
鞣花酸
高效液相色谱切换波长法
mouth rinse residue
drying method
gallic acid
methyl gallate
ellagic acid
high performance liquid chromatography(HPLC)switching walvelength
