摘要
目的:研究益生菌双歧杆菌V9(V9)对高脂饮食诱导的非酒精性脂肪肝(NAFLD)大鼠肝功能、氧化应激及脂代谢的影响,并探讨其作用机制。方法:雄性Wistar大鼠,随机分为空白组(Control)、高脂饮食组(HFD)、黄连素(BR)组(HFD+BR)、益生菌V9治疗组(HFD+V9)以及益生菌V9对照组(V9)。除Control组和V9组,其余组给予高脂饮食,6周后将高脂饮食换为普通饲料并进行灌胃给药,黄连素300 mg/kg、益生菌V91×109CFU/ml,4周后收集血清和肝脏组织。结果:与Control组相比,HFD组的AST、ALT、TC、MDA水平显著上升(P<0.05),SOD和SOD2的表达量显著下降(P<0.05),PPAR-α、PPAR-γ的表达受到抑制(P<0.05);与HFD组相比,HFD+V9及Berberine组的ALT、AST、TC、MDA水平显著降低(P<0.05),SOD活力、SOD2以及PPAR-α、PPAR-γ的表达量显著上升(P<0.05)。结论:益生菌V9可以通过抑制氧化应激反应、缓解脂代谢紊乱,改善高脂饮食诱导的NAFLD。
Objective:To study the effects of Probiotic Bifidobacterium V9 on liver function,oxidative stress and lipid metabolism response of Non-alcoholic fatty liver disease(NAFLD)rats and explore its mechanism.Methods:Wistar rats were randomly divided into Control group(Control),model group(HFD),Berberine group(HFD+BR),Probiotics V9 treatment group(HFD+V9)and Probiotics V9 control group(V9).Except for the Control group and the V9 group,the other groups were given high-fat diet.After 6 weeks,the high-fat diet was changed into normal diet and the drug was given by gavage,Berberine 300 mg/kg,probiotic V91×109 CFU/ml,serum and liver were collected after 4 weeks.Results:Compared with the Control group,the levels of AST,ALT,MDA and TC in the HFD group were increased(P<0.05),and the expression levels of SOD and SOD2 were decreased(P<0.05),the expression levels of PPAR-αand PPAR-γwere inhibited(P<0.05).Compared with the HFD group,the expression levels of ALT,AST,MDA,TC were decreased in Berberine and HFD/V9 groups(P<0.05),and the expression levels of SOD and SOD2 were increased(P<0.05),the expression levels of PPAR-αand PPAR-γwere significantly increased(P<0.05).Conclusion:Probiotics V9 can improve NAFLD in rats by inhibiting oxidative stress and alleviating lipid metabolism disorders.
作者
李欣益
刘春妍
黄建
颜妍
谢基明
王玉珍
LI Xin-Yi;LIU Chun-Yan;HUANG Jian;YAN Yan;XIE Ji-Ming;WANG Yu-Zhen(College of Life Science,Inner Mongolia Agricultural University,Hohehot 010018,China)
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2019年第23期2822-2826,共5页
Chinese Journal of Immunology
基金
国家自然科学基金(81560677)
内蒙古自然科学基金(2016JQ08,2016MS0836)的资助
