摘要
采用UASB连续流反应器,研究了不同有机物浓度对厌氧氨氧化的脱氮性能及微生物群落结构的长期影响,结果表明,在COD浓度分别为0,20,40,60和80mg/L时,40mg/L COD浓度条件下对厌氧氨氧化反应的促进程度最大,TN和COD去除率稳定在88.5%和75.3%.在低浓度COD(20mg/L)条件下,厌氧氨氧化反应受影响程度不明显,而COD为60和80mg/L时,系统脱氮性能受到不同程度的抑制.通过高通量测序技术对不同COD浓度下的微生物群落结构进行分析,结果表明不同COD浓度下,绿曲挠菌门(Chloroflexi)、浮霉菌门(Planctomycetes)、变形菌门(Proteobacteria)和放线菌门(Actinobacteria)等占据主导,且随着COD浓度从0增至80mg/L,浮霉菌门相对丰度从24.60%降至7.70%,其中的Candidatus Brocadia属降幅最大,丰度从12.14%减至3.63%,变形菌门相对丰度从15.40%增至36.30%,其中Bdelloribrio菌属的增幅最大,丰度从0.01%增至8.39%.
The UASB continuous flow reactor was used to study the long-term effects of different organic concentrations on the anammox nitrogen removal and microbial community structure.The results showed that the 40 mg/L of COD concentration provided the most promotion to anammox reaction among the COD concentration levels with 0,20,40,60 and 80 mg/L.At this concentration,the TN and COD removal rates were stabilized at 88.5% and 75.3%,respectively.Under a lower level(20 mg/L),the anammox reaction was not affected significantly.However,the nitrogen removal performance was inhibited when the COD concentrations were higher at 60 and 80 mg/L.The microbial community structure under different COD concentrations was analyzed by high-throughput sequencing technology.The results indicated that the Chloroflexi,Planctomycetes,Proteobacteria and Actinobacteria were always dominated under different COD concentrations,their relative abundance varied in different scenarios.The relative abundance of Planctomycetes was decreased from 24.60% to 7.70% with the increase of COD concentration(0 to80 mg/L).Among them,Candidatus Brocadia declined fastest,and the abundance was decreased from 12.14% to 3.63%.The relative abundance of Proteobacteria was increased from 15.40% to 36.30%,among which Bdelloribrio had the biggest increase from 0.01%to 8.39%.
作者
陈重军
汪瑶琪
姜滢
郭萌蕾
谢嘉玮
谢军祥
沈耀良
CHEN Chong-jun;WANG Yao-qi;JIANG Ying;GUO Meng-lei;XIE Jia-wei;XIE Jun-xiang;SHEN Yao-liang(School of Environmental Science and Engineering,Suzhou University of Science and Technology,Suzhou 215009,China;Jiangsu Provincial Key Laboratory of Environmental Science and Engineering,Suzhou 215009,China;Jiangsu Collaborative Innovation Center of Technology and Material of Water Treatment,Suzhou 215009,China;Jiangsu Key Laboratory of Anaerobic Biotechnology,Wuxi 214122,China)
出处
《中国环境科学》
EI
CAS
CSCD
北大核心
2019年第12期5049-5055,共7页
China Environmental Science
基金
国家自然科学基金项目(51508366,51308367)
江苏省环境科学与工程重点实验室开放基金(Zd1804)
江苏省厌氧生物技术重点实验室开放基金(KFLAB201701)
关键词
厌氧氨氧化
有机物浓度
脱氮性能
微生物群落结构
高通量测序
anammox
organic matter concentration
nitrogen removal
microbial community
high throughput sequencing
