摘要
为解决目前光片荧光显微镜光片厚度单一的问题,基于变倍扩束原理进行了可变光片照明系统设计。首先,对光片照明系统各组成部分进行了高斯光学计算,得到光片厚度与扩束比的关系以及扩束比与各组元垂轴放大率、焦距的关系;然后,设计了基于10倍扩束的可变光片照明系统,得到厚度和长度连续可变的光片;最后,对光片参数、均匀性及系统的公差进行分析。设计结果表明,连续可变光片的厚度为3.33~33.3μm,在YOZ平面上60%的光片高度区域内,低(1×)、中(6×)和高(10×)扩束比下的照度均匀性分别达到0.65、0.4和0.61。公差分析表明,光片厚度在1×扩束比时的最大改变量小于设计值的15%,在6×和10×扩束时小于6%。设计实现了光片厚度的连续变化,且在60%的光片高度区域内有利于样本的观察。
To solve the problem of the single thickness of the light-sheet fluorescence microscopy,the design of the variable light-sheet illumination system was carried out based on zoom beam expanding.First,each group of the light-sheet illumination system was calculated using Gaussian optics to obtain the relation between the thickness of the light-sheet and the expansion ratio as well as the relation among the expansion ratio,the lateral magnification and focal length of each group.Then a variable light-sheet illumination system based on 10×beam expanding was designed to generate the light-sheet with continuously variable thickness and length.Finally,the parameters and uniformity of the light-sheet and the system tolerance were analyzed.The results show that the thickness of the continuously variable light-sheet is 3.33μm to 33.3μm,and the illumination uniformity is 0.65,0.4 and 0.61 on the YOZ plane within 60%light-sheet height at low(1×),medium(6×)and high(10×)expansion ratios,respectively.Tolerance analysis shows that the maximum change of the light-sheet thickness is less than the 15%of design value at expansion ratio of 1×,less than 6%at 6×and 10×.The design realizes the continuous variation of the light sheet thickness,and the region of 60%light-sheet height is favorable for the observation of the sample.
作者
姜连
芮丛珊
曾春梅
马锁冬
Jiang Lian;Rui Congshan;Zeng Chunmei;Ma Suodong(School of Optoelectronic Science and Engineering,Soochow University,Suzhou 215006,China;Key Lab of Advanced Optical Manufacturing Technologies of Jiangsu Province,Key Lab of Modem Optical Technologies of Education Ministry of China,Suzhou 215006,China)
出处
《红外与激光工程》
EI
CSCD
北大核心
2019年第11期183-191,共9页
Infrared and Laser Engineering
基金
江苏高校优势学科建设工程资助项目
关键词
光学设计
可变光片照明系统
无焦变倍扩束
照度均匀性
optical design
variable light-sheet illumination system
afocal zoom expanding
illumination uniformity
