摘要
【目的】持续低温或阴雨天气对黑水虻成虫产卵量造成较大影响,在产卵高峰期冷藏多余虫卵用于卵量低谷期,将有利于规模化标准化生产黑水虻。【方法】研究了不同发育阶段黑水虻虫卵经12℃冷藏不同天数后的孵化情况,以及原肠期虫卵经12℃分别冷藏5、10、15 d后在32℃下孵化生长发育情况。【结果】初产虫卵不能直接冷藏,12℃冷藏1 d后转32℃孵化,死亡率达100%。胚盘胚带形成期、原肠期、体壁背合期和破壳期的虫卵在12℃下冷藏10 d转置32℃孵化,平均孵化率分别为94%、94%、27%、0%,体壁背合期和破壳期虫卵不适宜冷藏。胚盘胚带形成期虫卵冷藏13 d后孵化率降至80%以下;原肠期虫卵冷藏15 d后孵化率降至80%以下。冷藏5、10 d原肠期虫卵孵化的幼虫生长发育与对照无显著差异;冷藏15 d原肠期虫卵存活率低于对照,百头虫重无显著差异。【结论】在12℃冷藏的原肠期虫卵能保持较高的存活率,幼虫生长发育正常,该结果为黑水虻的工厂化生产提供理论依据与实践指导。
【Objective】The egg production of Hermetia illucens L.is greatly affected by continuous low temperature or rainy weather.It is helpful to standardize the production of H.illucens by refrigerating the surplus eggs during the peak spawning period.【Method】In this paper,the egg hatching rate of H.illucens at different developmental stages after being refrigerated at 12℃ for different days,and the hatching,growth and development of eggs at gastrula stage at 32℃ after being refrigerated at 12℃ for 5,10 and 15 days respectively was studied.【Result】The results showed that the primiparous eggs could not be refrigerated directly.Refrigerated at 12℃ for 1 day and then incubated at 32℃,the mortality rate of eggs was 100%.The eggs at blastoderm and germ band formation stage,gastrula stage,dorsal closure stage and hatching stage were refrigerated at 12℃ and then incubated at 32℃.The average hatching rate were 94%,94%,27% and 0%,respectively.Therefore,the eggs at dorsal closure stage and hatching stage were not suitable for cold storage.The hatching rate of eggs during blastoderm and germ band formation stage was reduced to less than 80% after 13 days of cold storage.The hatching rate of the eggs during gastrula stage was reduced to less than 80% after 15 days of cold storage.There were no significant difference in the growth and development between the larvae hatched from the eggs at gastrula stage that were refrigerated for 5 and 10 days and the larvae of control group.The survival rate of the eggs at gastrula stage which were refrigerated for 15 days was lower than that of the control group.There were no significant difference in the weight of one hundred larvae.【Conclusion】The eggs at gastrula stage refrigerated at 12℃ can maintain a high survival rate,and the larvae can grow and develop normally.The results can be used to provide theoretical basis and practical guidance for industrial production of H.illucens.
作者
王凤英
肖乾鹏
王星
WANG Fengying;XIAO Qianpeng;WANG Xing(Guangzhou Outreach Environmental Technologies Co.,Ltd.,Guangzhou 510450,China;College of Plant Protection,Hunan Agricultural University,Changsha 410128,China)
出处
《广东农业科学》
CAS
2019年第10期82-86,共5页
Guangdong Agricultural Sciences
关键词
黑水虻
虫卵
冷藏保存
孵化率
幼虫生长发育
Hermetia illucens L.
eggs
cold storage
hatching rate
larval growth and development
