摘要
Adva nces in genome editi ng tech no logy have revoluti on ized basic and applied biology research in recent years,particu?larly due to the newly emerged CRISPR/Cas technique(Ren et al.,2017b).The classical CRISPR/Cas system was derived from a bacterial defense system,which consists of a single guide RNA(sgRNA)for precise targeting and one Cas protein for DNA binding and nuclease activity.Relying on high precision of CRISPR/Cas system,multiple functions have been exploited in addition to original nuclease activity,such as genome base editing,gene knock-down and activation,chromatin imaging systems etc.
基金
This work was supported by Genome Tagging Project and grants from the Chinese Academy of Sciences(XDB19010204)
Shanghai Municipal Commission for Science and Technology(17411954900,17JC1400900,17JC1420102,16JC420500)
the National Natural Science Foundation of China(3153004&81672117,31730062 and 31821004).
