摘要
目的·研究大鼠深Ⅱ度灼伤后皮肤力学性质的改变及变性真皮脱细胞后用于复合移植再利用的可行性。方法·建立SD大鼠背部深Ⅱ度灼伤模型,灼伤后3 h及3 d后切取皮肤组织,利用力学检测器,检测皮肤弹性模量及最大载荷;另外,制作正常皮肤及灼伤后3 h皮肤的脱细胞真皮基质,比较真皮基质的结构差异以及进行异体复合移植,观察移植成活皮片的大体及组织学结构。结果·大鼠正常皮肤、灼伤后3 h及3 d皮肤的弹性模量分别是(8.7±6.4)MPa、(7.2±2.7)MPa和(2.7±0.3)MPa,最大载荷分别是(107.1±41.7)N、(81.5±10.8)N和(42.2±7.0)N;灼伤后3 d皮肤的弹性模量和最大载荷均较正常组显著降低(均P<0.05)。灼伤后3 h的变性脱细胞真皮基质仍保留了与正常真皮大致相近的孔隙结构;将变性脱细胞真皮基质进行复合移植,愈后外观和组织学结构与对照组基本相似。结论·深Ⅱ度灼伤早期变性皮肤组织与正常皮肤的力学性质相近,组织结构相似,可作为脱细胞真皮基质的潜在来源进行复合移植。
Objective·To investigate the changes of skin mechanical properties after deep second-degree burn,and study the feasibility of composite transplantation by using denatured dermal acellular matrix.Methods·The deep second-degree burn models in SD rats were constructed,and then the full skins were harvested after 3 h and 3 d,respectively,to compare the skin modulus of elasticity and ultimate load with normal skins.In addition,the structural differences of the dermal matrix were also compared.The composite transplantations were conducted by using the normal or denatured dermal acellular matrix,and then the general and histological structures of living skin grafts were observed.Results·The moduli of elasticity of the normal skins and the skins 3 h and 3 d after burn in SD rats were(8.7±6.4)MPa,(7.2±2.7)MPa and(2.7±0.3)MPa,respectively.The ultimate loads were(107.1±41.7)N,(81.5±10.8)N and(42.2±7.0)N,respectively.Both indexes were significantly reduced in the skins 3 d after burn compared to the normal ones(P<0.05).In the acellular matrix of denatured dermis 3 h after burn,the pore structure was still similar to that of normal one,and the appearance and histological structure after healing from composite transplantation were similar to those of the control group.Conclusion·The early denatured skin tissue after deep second-degree burn maintains the similar mechanical properties and histological structure to the normal skin,which is a potential source of acellular dermal matrix for composite transplantation.
作者
王志勇
王西樵
刘英开
董叫云
宋菲
曹晓赞
陆树良
WANG Zhi-yong;WANG Xi-qiao;LIU Ying-kai;DONG Jiao-Yun;SONG Fei;CAO Xiao-zan;LU Shu-liang(Department of Burns and Plastic Surgery,Ruijin Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 200025;Shanghai Burns Institute,Shanghai 200025)
出处
《上海交通大学学报（医学版）》
CAS
CSCD
北大核心
2019年第11期1239-1242,共4页
Journal of Shanghai Jiao tong University:Medical Science
基金
国家自然科学基金(81671914,81071567,30872686,81000838)~~
关键词
灼伤
变性真皮
生物力学
脱细胞真皮基质
复合移植
burn
denatured dermis
biomechanics
acellular dermal matrix
composite transplantation
