摘要
【目的】探讨肝细胞癌(HCC)超氧化物歧化酶铜伴侣蛋白(CCS)表达水平与肿瘤恶性生物学指标之间的相关性。【方法】收集2018年1月至2018年12月在中山大学附属第一医院行外科切除的10例肝细胞癌肿瘤标本以及患者的临床及病理资料,采用蛋白免疫印迹法(WB)检测肝癌及相应癌旁组织CCS表达水平,采用免疫组化(IHC)染色法检测Ki67,CD34,vimentin及glypican-3(GPC3)表达情况。用Wilcoxon秩和检验法分析CCS表达水平与各肝细胞癌恶性相关生物学指标Ki67,CD34,vimentin及GPC3表达水平之间的关系;采用Fisher精确概率法分析CCS表达水平与肝细胞癌患者临床及病理特征之间的关系。【结果】10例肝癌患者的肝癌组织标本中,7例CCS表达水平较癌旁组织低(7/10),3例CCS表达水平较癌旁组织高(3/10)。肝细胞癌CCS低表达组的Ki67,vimentin及GPC3表达水平高于CCS高表达组(Z=-2.400,P=0.016;Z=-2.423,P=0.015;Z=-2.400,P=0.016);肝细胞癌CCS低表达组CD34表达水平低于CCS低表达组(Z=-2.423,P=0.015);CCS高表达组与低表达组之间在年龄、性别、乙肝病史、肝硬化、术前AFP水平、肿瘤大小、ES分级、微血管侵犯各临床及病理特征方面均未呈现显著统计学差异。【结论】本研究显示多数肝细胞癌的CCS表达水平低于相应癌旁组织,且与CCS高表达的肝细胞癌相比,CCS低表达的肝细胞癌Ki67,vimentin及GPC3表达水平较高,提示CCS表达降低与肝细胞癌增殖、侵袭、转移等恶性生物学行为相关。CCS低表达组CD34表达水平低于CCS高表达组,但其相关机制有待于进一步探究。本研究结果显示,与正常肝脏组织相比,CCS在肝癌中的表达多呈下降趋势,其表达水平可能成为肝细胞癌治疗预后的预测指标。
【Objective】To explore the correlation between the expression levels of copper chaperone for superoxide dismutase(CCS)and the malignancy related biomarkers in hepatocellular carcinoma(HCC).【Methods】From January to December 2018,we obtained fresh samples of surgically dissected HCC paired with para-carcinoma normal tissues from10 HCC patients and collected their clinical and pathological data. Western blotting(WB)was performed to examine the expression of CCS in HCC tissues and adjacent noncancerous tissues. Immunohistochemical staining(IHC)was employed to detect the expression of Ki67,CD34,vimentin and glypican-3(GPC3). The correlation between the expression levels of CCS and biomarkers was analyzed by using Wilcoxon rank sum test. The association between CCS expression and clinical pathological characteristics of HCC patients was investigated by using Fisher′s exact probability test.【Results】In 7 of the 10 HCC cases,the expression level of CCS in HCC tissue samples was lower than that in adjacent noncancerous tissues,and in other 3 HCC cases,CCS expression higher. In the group with low CCS expression,compared with those in the group with high CCS expression,the expression levels of Ki67,vimentin and GPC3 were higher(Z=-2.400,P=0.016;Z=-2.423,P=0.015;Z=-2.400,P=0.016),while the expression level of CD34 lower(Z=-2.423,P=0.015).There was no statistically significant difference in clinical and pathological variables including gender,age,hepatitis B virus infection,liver cirrhosis,preoperative serum AFP level,tumor size,Edmondson-Steiner grade and microvascular invasion between two groups with high and low CCS expression.【Conclusions】The results revealed that in most of HCC patients,the expression level of CCS in HCC tissues was lower than that in adjacent noncancerous tissues. Additionally,higher expression levels of Ki67,vimentin and GPC3 in HCC tissues with low CCS expression indicated that low expression level of CCS correlated with malignant biological behaviors such as HCC proliferation,invasio
作者
温春勇
孙文静
万源
林润
李楠
山长亮
杨建勇
黄勇慧
WEN Chun-yong;SUN Wen-jing;WAN Yuan;LIN Run;LI Nan;SHAN Chang-liang;YANG Jian-yong;HUANG Yong-hui(Department of Interventional Radiology,The First Affiliated Hospital,Sun Yat-sen University,Guangzhou 510080,China;Biomedical Translational Research Institute,Jinan University,Guangzhou 510632,China)
出处
《中山大学学报（医学版）》
CAS
CSCD
北大核心
2019年第6期889-896,共8页
Journal of Sun Yat-Sen University:Medical Sciences
基金
国家自然科学基金(81402504)
