摘要
以处理实际生活污水的连续流MUCT工艺为基础,探究了全程硝化和短程硝化状态下优势聚磷菌Candidatus Accumulibacter的群落动态与系统反硝化除磷效果之间的相互联系.流式细胞仪定量检测结果表明,系统中聚磷菌占全菌的比例为22.1%,Accumulibacter是处于全程硝化状态下的优势聚磷菌,对系统中氮磷的去除起到重要作用.q PCR定量结果表明,短程硝化状态下的优势种IIC和IIC(ppk1 excluding OTU NS D3)以及IIF的增长率较快,占总Accumulibacter比例分别为11.53%、34.31%和17.97%,其利用亚硝进行反硝化除磷的能力比其他分支强,对系统化中亚硝的去除起到重要作用.IID分支在反应器整个运行阶段一直处于优势地位,是系统内氮磷去除最重要的分支.其在全程硝化状态下占Accumulibacter分支总和的80%,但在短程状态下的竞争能力不如其他的TypeⅡ型分支.
In this study,Modified University of Cape Town(MUCT)process was used to treat domestic sewage with lowρ(C)/ρ(N)ratio,and the relationship between population dynamics of dominant Candidatus Accumulibacter and efficiency of denitrifying phosphorus removal under nitration and nitrification was studied.Flow cytometry based quantification showed that Accumulibacter accounting for22.1%of total bacteria were dominant phosphate accumulating organisms(PAOs)in system under nitrification.qPCR based estimation indicated that dominant species IIC,IIC(ppk1 excluding OTU NS D3)and IIF grew fast and accounted for 11.53%,34.31%and 17.97%of Accumulibacter,respectively,while their ability using nitrite to achieve denitrifying phosphorus removal were stronger than that of other clades and were important for nitrite removal in system.As the most important clade in N and P removal in system,IID was always dominant along entire operation,which accounted for 80%of Accumulibacter under nitrification,but not as competitive as other TypeⅡclades under nitration.
作者
曾薇
李超
孟庆安
马晨阳
ZENG Wei;LI Chao;MENG Qing'an;MA Chenyang(National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology,Engineering Research Center of Beijing for Denitrifying Phosphorous Removal and Process Control,Beijing 100124,China)
出处
《北京工业大学学报》
CAS
CSCD
北大核心
2019年第12期1243-1251,共9页
Journal of Beijing University of Technology
基金
国家自然科学基金资助项目(51578016)
