摘要
目的使用微量稀释法和E-test法分析肠杆菌科细菌对多黏菌素B体外敏感性,评估E-test法多黏菌素B药敏条在临床药敏检测中的价值。方法收集了887株临床肠杆菌科细菌,同时采用E-test法和微量稀释法进行体外药敏试验,考察两种方法的一致性,针对不一致的进行复测,复测仍不一致者判为不一致,对两种检测方法进行统计学分析。结果887株菌的平行检测中,842株用药敏条和微量稀释法测定MIC值在|±1|个稀释倍数的范围内,有45株菌的最低抑菌浓度(minimal inhibitory concentration,MIC)值超出了此范围,复测有8株菌的MIC值回到了|±1|个稀释倍数的范围内,即纠正后的EA(essential agreement)%为95.94%(851/887)。肠杆菌科内按照种属分析,EA值最高的为沙门菌属的97.09%(100/103),EA最低的为其他属肠杆菌的94.03%(126/134),统计学分析,不同属间无显著性差异。结论多黏菌素B药敏条用于肠杆菌科细菌检测药敏检测所测定的MIC值判定结果准确可靠,与CLSI推荐的微量稀释法的符合率在94%以上,能满足临床和实验室药敏检测需求。
Objective To analyze the sensitivity of Enterobacteriaceae bacteria to polymyxin B in vitro by the micro-dilution method and the gradient diffusion method,and evaluate the value of polymyxin B susceptibility strip in clinical drug sensitivity test.Methods A total of 887 clinical Enterobacteriaceae bacteria were collected.The gradient diffusion method and the micro-dilution methods were used to conduct in vitro susceptibility determination.The consistency of the two methods was investigated.The inconsistency was retested,and the retest was still inconsistent.Statistical analysis was performed on the two detection methods.Results In the parallel detection of 887 strains,842 strains were determined by the drug sensitivity strip and the micro-dilution method within|±1|dilution multiple range,and 45 beyond|±1|dilution multiple range.Eight strains returned to within|±1|dilution multiple range,meaning the corrected EA(essential agreement)was 95.94%(851/887).According to the species analysis,the highest EA value was 97.09%(100/103)of Salmonella,and the lowest EA was 94.03%(126/134)of other Enterobacteriaceae.Statistical analysis showed no significant differences between the different genera.Conclusion The results of MIC determination of polymyxin B susceptibility strip for the detection of susceptibility of Enterobacteriaceae bacteria were accurate and reliable,and the coincidence rate with CLSI recommended micro-dilution method was above 94%,which might meet the requirements of clinical and laboratory drug sensitivity tests.
作者
郑业焕
李轶
金湘东
荆鹏伟
Zheng Ye-huan;Li Yi;Jin Xiang-dong;Jing Peng-wei(Zhengzhou Autobio Diagnostics Co.,Ltd,Zhengzhou 450016;Henan Provincial People's Hospital,Zhengzhou 450003;Zhengzhou Railway Vocational and Technical College,Zhengzhou 450052;First Affiliated Hospital of Henan University of Traditional Chinese Medicine,Zhengzhou 450008)
出处
《中国抗生素杂志》
CAS
CSCD
2019年第11期1312-1315,共4页
Chinese Journal of Antibiotics
基金
河南地区多中心碳青霉烯类耐药肠杆菌中多黏菌素耐药机制研究(No.SBGJ2018084)
自动化微生物鉴定和药敏分析工作站开发及产业化应用(No.181200211900)
关键词
E-TEST法
微量稀释法
一致率
肠杆菌
Gradient diffusion method
Micro-dilution method
Concordance rate
Enterobacteriaceae
