摘要
目的观察红参水提物对小鼠免疫功能的影响。方法经水提取浓缩制得红参水提物,10 ml水提物相当于2.86 g的红参原材。将240只小鼠按随机数字表法分为空白对照组及红参水提物低、中和高剂量组,每组60只。红参水提物低、中、高剂量组小鼠灌胃红参提取物0.24、0.48、1.43 g/(kg·d),空白对照组灌胃等体积去离子水,按0.1 ml/10 g小鼠体重灌胃,1次/d,连续灌胃30 d。采用脏器/体重比值测定、迟发型变态反应实验、刀豆蛋白A诱导的小鼠淋巴细胞转化实验、抗体生成细胞实验、半数溶血值(half hemolytic value,HC50)实验、碳粒廓清实验、巨噬细胞吞噬鸡红细胞实验和自然杀伤细胞(natural killer cell,NK)细胞活性实验,观察红参水提物的免疫调节作用。结果与空白对照组比较,红参水提物低、中剂量组足趾肿胀度[(0.62±0.14)mm、(0.53±0.12)mm比(0.36±0.10)mm]升高(P<0.05),红参水提物低、高剂量组淋巴细胞增殖能力[(0.173±0.054)、(0.189±0.063)比(0.098±0.012)]升高(P<0.05);红参水提物中、高剂量组溶血空斑数[(137.49±24.73)×10^3/全脾、(148.43±27.53)×10^3/全脾比(112.96±26.28)×10^3/全脾]增多(P<0.05或P<0.01);红参水提物不同剂量组吞噬率[(35.67±3.82)%、(49.26±6.54)%、(57.92±7.36)%比(24.34±4.22)%]、吞噬指数[(0.72±0.23)、(0.82±0.15)、(0.91±0.26)比(0.35±0.11)]升高(P<0.01),但其对小鼠脾脏/体重比值、胸腺/体重比值、半数溶血值、碳廓清功能和NK细胞活性无显著影响。结论红参水提物可增强小鼠的免疫功能。
Objective To investigate the immune enhancement effect of red ginseng aqueous extract in mice.Methods The aqueous extract of red ginseng was obtained by water extraction and concentration,and the aqueous extract 10 ml was equivalent to raw material 2.86 g.The experiment was divided into the blank control group,low dose group of red ginseng aqueous extract 0.24 g/(kg d),middle dose group 0.48 g/(kg d)and high dose group 1.43 g/(kg d).In order to explore the immunoregulation effect of red ginseng aqueous extract,Organ/body weight ratio measurement,the delayed type hypersensitivity(DTH)reaction,the spleen lymphocyte transformation experiment induced by Con A,the detection of antibody-producing,the determination of serum hemolysin experiments,the clearance rate of carbon particles,the phagocytosis effect of macrophages with chicken red blood cell experiments,and the determination of natural killer cell activity were carried out with the mice.Results Compared to the blank control group,the tow edema degree(0.62±0.14 mm,0.53±0.12 mm vs.0.36±0.10 mm)of low dose group and medium dose group significantly increased(P<0.05).The ability of proliferation of T lymphocytes(0.173±0.054,0.189±0.063 vs.0.098±0.012)of low dose group and high dose group significantly increased(P<0.05).The number of haemolytic empty spots(137.49×10^3±24.73×10^3,148.43×10^3±27.53×10^3vs.112.96×10^3±26.28×10^3)of medium dose group and high dose group significantly increased(P<0.05).The phagocytosis rate(35.67%±3.82%,49.26%±6.54%,57.92%±7.36%vs.24.34%±4.22%)and index(0.72±0.23,0.82±0.15,0.91±0.26 vs.0.35±0.11)of low-,medium-,high-dose group significantly increased(P<0.05).However,there was no statistical difference in the organ/body weight ratio of the experimental animals,the determination of serum hemolysin experiments,the clearance rate of carbon particles,and NK cells activity.Conclusion Red ginseng aqueous extract has an exact role in immunity improvement in mice.
作者
张方毅
郭遂成
Zhang Fangyi;Guo Suicheng(Department of Traditional Chinese Medicine,Nanyang Medical College,Nanyang 473061,China;Department of Estive Medical,First Affiliated Hospital of Nanyang Medical College,Nanyang 473058,China)
出处
《国际中医中药杂志》
2019年第11期1219-1224,共6页
International Journal of Traditional Chinese Medicine
基金
南阳市科技攻关项目(KGGG2018107)。
关键词
人参
红参
免疫调节
小鼠
Ginseng radix et rhizoma
Ginseng radix et rhizoma rubra
Immunoregulation
Mice
