摘要
目的 优化酶解黑芥子苷转化成异硫氰酸烯丙酯(allyl isothiocyanate,AITC)的水解条件.方法 在已知AITC的测定条件下,以AITC转化率为考察指标,对温度、pH、Zn^2+离子添加量、乙二胺四乙酸(ethylene diamine tetraacetic acid,EDTA)添加量及酶来源进行单因素试验以及设计五因素四水平的正交试验,确定最优转化工艺条件.结果 黑芥子苷对AITC最优转化条件为:黑芥子酶来源为樱桃萝卜种子、pH为7.00、Zn^2+的添加量为1.00 mmol/L、转化温度为40℃、螯合EDTA添加量为0.30 mmol/L.最优转化条件下AITC的转化率为70.32%.结论 本研究的实验设计简单且能真实的提高AITC的转化率,对酶解法制备AITC提供数据依靠.
Objective To optimize the hydrolysis condition for allyl isothiocyanate(AITC) transformed from myrosinase. Methods The transformation rate of AITC was taken as the index under the condition of known determination of AITC. Single factor experiments(temperature, pH, additive amount of Zn^2+, additive amount of EDTA and resource of myrosinase) and 5 factors and 4 levels orthogonal experiments were designed to determine the optimal enzymatic hydrolysis conditions. Results The optimum transformation conditions for enzymatic hydrolysis of myroside to AITC were as follows: myrosinase originated from cherry radish seeds, pH 7.00, Zn^2+ 1 mmol/L, temperature 40 ℃, EDTA dosage 0.30 mmol/L. The conversion of AITC was 70.32% under the optimal hydrolysis conditions. Conclusion The experimental design of this study was simple and could actually improve the conversion rate of AITC, which provided data support for the preparation of AITC by enzymatic hydrolysis.
作者
丁瑞霞
靳方舟
丁轲
韩涛
陈湘宁
DING Rui-Xia;JIN Fang-Zhou;DING Ke;HAN Tao;CHEN Xiang-Ning(College of Food Science and Engineering,Beijing University of Agriculture,Zhongguancun Agricultural Biological Products and Seed Industry Open Laboratory,Beijing 102206,China;Microbiological and Safety Inspection and Control of Pesticide Residues in Agricultural Products HarmfulBeijing Key Laboratory,Beijing 102206,China)
出处
《食品安全质量检测学报》
CAS
2019年第21期7336-7342,共7页
Journal of Food Safety and Quality
基金
北京市自然科学基金委员会——北京市教育委员会联合资助项目(KZ201710020014)~~
