摘要
以油茶茶籽粕为原料,采用乙醇水溶液提取茶皂素。在茶籽粉和乙醇料液比1∶9(g∶mL),乙醇体积分数60%,提取温度60℃和提取时间3 h的最佳条件下茶皂素的提取得率达14.9%。用NKA-9型大孔吸附树脂吸附纯化茶皂素粗品,树脂静态吸附与解吸结果表明:树脂静态吸附茶皂素粗提液0.5 h基本饱和,体积分数80%乙醇解吸率为91.1%;动态吸附与解吸时,上样流速8 mL/min较佳,吸附率为66.04%,体积分数80%乙醇洗脱,洗脱流速5.0 mL/min,洗脱体积50 mL时,可使流出液中茶皂素质量浓度在1.25~1.57 g/L之间,茶皂素纯度为95%。
Tea saponin was extracted by alcohol extraction using tea seed meal as raw material.The optimum extraction conditions of tea saponin was as follows:the ratio of solid to liquid was 1∶9(g/mL),the volume fraction of extraction solution(ethanol)was 60%,the extraction temperature was 60℃and the extraction time was 3 h,under the above reaction conditions,the highest yield of tea saponin was 14.9%.Crude tea saponin was purified by NKA-9 macroporous resin.In the purification experiment,the static adsorption reached saturation for 0.5 h and the desorption rate of the volume fraction 80%ethanol reached the maximum of 91.1%in the static desorption,while the dynamic adsorption and desorption.The adsorption rate reached 66.04%at the rate of 8 mL/min,and the concentration of tea saponin was between 1.25 g/L and 1.57 g/L after elution with the volume fraction 80%ethanol of 50 mL at the flow rate of 5.0 mL/min,tea saponin purity 95%.
作者
孙万里
SUN Wanli(College of Chemistry and Bioengineering,Yichun University,Yichun 336000,China)
出处
《生物质化学工程》
CAS
北大核心
2019年第6期39-44,共6页
Biomass Chemical Engineering
基金
宜春学院地方发展研究中心招标项目(2210816026)
