摘要
【目的】分析绣球菌水溶性多糖的结构与免疫活性。【方法】采用热水提取法从广叶绣球菌Sparassis latifolia的真空冷冻干燥品中提取得到绣球菌水溶性多糖SCG-D,然后通过DEAE Sepharose Fast Flow离子交换层析对其进行分离,获得SCG-N和SCG-A两个多糖组分,并对SCG-A组分进行HPSEC、单糖组成分析、红外光谱、1HNMR和13C-NMR核磁共振的结构分析。通过大鼠脾淋巴细胞体外刺激活性测试比较了SCG-D及其组分SCG-A、SCG-N的体外免疫活性差异。【结果】绣球菌水溶性多糖SCG-D具有促进鼠脾淋巴细胞增殖的活性,其酸性多糖组分SCG-A促进作用最强,其100μg·mL^-1 72 h处理组的增殖率为32.7%,而SCG-N多糖组分的活性较SCG-A低,其100μg·mL^-1 72 h处理组增殖率为11.66%。结构分析结果表明SCG-A的重均分子量为4.30×10^5 Da,其主链结构为α-1,4-D-葡聚糖,主要由葡萄糖和半乳糖构成,并且还含有一定量的1-6分支结构。【结论】绣球菌冻干品的水溶性多糖及其组分具有促进大鼠脾淋巴细胞体外增殖的免疫活性,其中活性最强的酸性多糖组分SCG-A是一种具有1-6分支结构的α-1,4-葡聚糖。
【Objective】Chemical structure and in vitro immunological activity of the water soluble polysaccharides from Sparassis latifolia were studied.【Method】The lyophilized powder of S.latifolia fruiting bodies was extracted by hot water to obtain polysaccharides SCG-D.From the extract,the SCG-N and SCG-A fractions were isolated by the DEAE-Sepharose Fast Flow ion exchange chromatography.The structure of SCG-A was analyzed using HPSEC,monosaccharide composition analysis,FT-IR,and NMR.The in vitro immunological activities of the extract and the fractions were determined by a test on rat spleen lymphocytes.【Result】SCG-D was found active in promoting the lymphocyte proliferation.SCG-A exhibited the strongest activity with a proliferation rate of 32.7%at the concentration of 100μg/mL in 72h,while SCG-N activity was lower at 11.66%.The molecular weight of SCG-A was 4.30×10^5Da with anα-1,4-D-glucan as its main chain consisting primarily of glucose and galactose with some 1-6-branches.【Conclusion】The water-soluble polysaccharides and its fractions obtained from the lyophilized S.latifolia powder showed varying in vitro immune activities of promoting the proliferation of rat spleen lymphocytes.The acidic SCG-A had a main chain structure ofα-1,4-glucan with 1-6-branches and showed the greatest immunological activity among the 3 polysaccharide materials.
作者
张迪
王宏雨
肖冬来
林衍铨
ZHANG Di;WANG Hong-yu;XIAO Dong-lai;LIN Yan-quan(Institute of Edible&Medicinal Fungi,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350014,China)
出处
《福建农业学报》
CAS
CSCD
北大核心
2019年第9期1093-1099,共7页
Fujian Journal of Agricultural Sciences
基金
福建省科技计划公益类专项(2018R1020-3)
福建省财政专项——福建省农业科学院科技创新团队建设项目(STIT2017-1-6)
关键词
广叶绣球菌
多糖
脾淋巴细胞
免疫
Sparassis latifolia
polysaccharide
spleen lymphocyte
immunity
