摘要
苦荞(Fagopyrum tataricum)芽菜是一种新兴健康食品,该研究通过不同光周期(0、4、8、12、16和20 h·d^-1)处理苦荞芽菜,测定其生物量、叶绿素和主要营养成分的含量以及黄酮合成相关基因的表达水平,以明确不同光周期对苦荞芽菜品质的影响机理。结果表明:(1)随着苦荞芽菜生长时间的增加,芽菜生物量总体呈上升趋势,16 h·d^-1光周期时芽菜生长状态最好,鲜重最大。(2)不同光周期对苦荞芽菜各营养成分含量的影响有所差异,可溶性糖含量在芽菜萌发第2天开始逐渐下降,10 d后含量仅为第2天的13.5%~14.5%;花青素含量在芽菜萌发第2天光周期为12 h·d^-1时最高(2.16 mg/g),不同光周期处理4~10 d后均显示出降低趋势;芦丁是苦荞芽菜中主要的黄酮类化合物,芦丁含量在不同光周期处理后有所变化且适当的光照有利于其合成和积累,并在处理第4天光周期为16 h·d^-1时含量最高(59.60 mg/g)。(3)qRT-PCR分析表明,不同光周期处理的苦荞芽菜中各黄酮合成相关基因的表达量在第2天最高,随生长周期其表达量均不同程度降低,但黄酮醇合成支路关键酶基因FtFLS1和花青素支路基因FtDFR2表达量上升、且显示出强烈的光诱导特征。研究发现,光周期对苦荞芽菜的生物量影响较大,黑暗有利于胚轴伸长,长光周期(16 h·d^-1)有利于鲜重的增加;在苦荞萌发过程中,苦荞芽菜可溶性糖、花青素和芦丁含量整体呈减少趋势,可溶性糖和花青素含量均在光周期16 h·d^-1处理第2天时最高,芦丁含量在光周期为16 h·d^-1处理第4天时最高,建议苦荞芽菜在光周期为16 h·d^-1生长4 d时采食。
Tartary buckwheat(Fagopyrum tataricum) sprouts is a newly healthy food. Tartary buckwheat sprouts were treated with different photoperiod(0, 4, 8, 12, 16 and 20 h·d^-1). The biomass, the contents of chlorophyll and main nutritional components, and the expression level of genes related to flavonoids synthesis were measured in order to clarify the mechanism of the effect of different photoperiod on the quality of Tartary buckwheat sprouts, the results showed that:(1) with the increase of growth time of Tartary buckwheat sprouts, the biomass of Tartary buckwheat sprouts increased as a whole, and the growth state of sprouts was the best and the fresh weight was the highest at 16 h·d^-1 photoperiod.(2) The effects of different photoperiod on the contents of nutrients in Tartary buckwheat sprouts were different. The content of soluble sugar began to decrease gradually on the second day of germination, and the content after 10 days was only 13.5% and 14.5% of that on the second day. The anthocyanin content was the highest(2.16 mg·g^-1) when the photoperiod was 12 h·d^-1 on the second day of sprouting, and decreased after 4 to 10 days of different photoperiod treatments. Rutin is the main flavonoids in Tartary buckwheat sprouts. The content of rutin changed after different photoperiod treatments, and proper light was beneficial to its synthesis and accumulation. The highest content(59.60 mg·g^-1) was obtained when the photoperiod was 16 h·d^-1 on the 4 th day of treatment.(3) qRT-PCR analysis showed that the expression of flavonoids synthesis related genes in Tartary buckwheat sprouts treated with different photoperiod was the highest on the second day, and decreased with the growth cycle. However, the expression of the key enzyme gene FtFLS1 and the anthocyanin branch gene FtDFR2 increased, and showed strong light-induced characteristics. The results showed that photoperiod had a great effect on the biomass of Tartary buckwheat sprouts, darkness was beneficial to Hypocotyl elongation, and long photoperiod(16 h·d^
作者
吕兵兵
姚攀锋
王官凤
李成磊
赵海霞
王晓丽
L Bingbing;YAO Panfeng;WANG Guanfeng;LI Chenglei;ZHAO Haixia;WANG Xiaoli(College of Life Science,Sichuan Agricultural University,Ya an,Sichuan 625014,China)
出处
《西北植物学报》
CAS
CSCD
北大核心
2019年第10期1785-1794,共10页
Acta Botanica Boreali-Occidentalia Sinica
基金
国家自然科学基金(3187101578)
关键词
苦荞芽菜
光周期
黄酮醇
花青素
可溶性糖
tartary buckwheat sprouts
photoperiod
flavonoids
anthocyanins
soluble sugar
