摘要
目的探讨血清外泌体miR-92a在弥漫大B细胞淋巴瘤康艾注射液联合化疗治疗监测中的临床意义。方法选取42例弥漫大B细胞淋巴瘤患者为观察对象,按照治疗方法不同平均分为对照组和研究组。对照组患者采用CHOP方案化疗,研究组患者在对照组基础上联合康艾注射液治疗。采用realtime PCR法检测所有患者在治疗前后血清中外泌体miR-92a的表达水平;收集不同时间点血清评估miR-92a与治疗效果的关系。结果与对照组相比,研究组患者总有效率明显升高,组间比较差异具有统计学意义(P<0.05);研究组患者降低血清外泌体miR-92a的程度更为明显,组间比较差异具有显著性(P<0.05)。与化疗前相比,对照组和研究组均能明显降低患者血清外泌体miR-92a的表达水平,差异具有统计学意义(P<0.05)。弥漫大B细胞淋巴瘤患者miR-92a与c-MYC呈负相关关系(r=-0.451,P<0.001)。血清外泌体miR-92a区分缓解与非缓解患者的效能较强。结论血清外泌体miR-92a在弥漫大B细胞淋巴瘤患者中表达升高,其检测可用于监测康艾注射液联合化疗治疗的临床疗效。
Objective To investigate the clinical significance of serum exosome miR-92a in the monitoring of Kangai injection combined with chemotherapy for diffuse large B-cell lymphoma.Methods 42 patients with diffuse large Bcell lymphoma were enrolled in the study.They were divided into control group and study group according to different treatment methods.The control group was treated with CHOP chemotherapy,and the patients in the study group were treated with Kangai injection on the basis of treatment in the control group.The expression levels of exosome miR-92a in serum of all patients before and after treatment were detected by realtime PCR.The relationship between serum miR-92a and therapeutic effect was evaluated at different time points.Results Compared with that of the control group,the total effective rate of the study group was significantly higher,and the difference between the groups was statistically significant(P<0.05).Patients in the study group were more likely to reduce serum exosome miR-92a,and the difference between the two groups was significant(P<0.05).Compared with that before chemotherapy,both the control group and the study group significantly reduced the expression level of serum exosome miR-92a in patients after chemotherapy,and the difference was statistically significant(P<0.05).There was a negative correlation between miR-92a and c-MYC in patients with diffuse large B-cell lymphoma(r=-0.451,P<0.001).Serum exosome miR-92a had strong performance in distinguishing between patients with remission and non-remission.Conclusion The expression of serum exosome miR-92a is high in patients with diffuse large B-cell lymphoma.The detection can be used to monitor the clinical efficacy of Kangai injection combined with chemotherapy.
作者
王妃菲
张宇
吴筱莲
WANG Feifei;ZHANG Yu;WU Xiaolian(Department of Hematology,the First Affiliated Hospital of Zhejiang University of Traditional Chinese Medicine,Hangzhou310000,China)
出处
《中国现代医生》
2019年第30期42-45,I0002,共5页
China Modern Doctor
基金
浙江省医药卫生科技计划项目(2017KY116)