摘要
目的:通过病毒示踪技术,研究牙髓刺激相关开颌反射的大脑核团,尤其是间脑和边缘系统的神经核团。方法:通过运用顺行跨多突触病毒及逆行跨多突触病毒示踪开口肌运动相关核团及牙髓感觉相关核团。结果:小鼠全脑范围均出现荧光标记的神经元细胞,两种病毒共标记脑区12个,分布在端脑、间脑、边缘系统、中脑及网状结构。结论:开颌反射由多个核团参与,涉及全脑多个脑区,包括位于间脑的旁丘脑底核、下丘脑旁底核后部、未定带和边缘系统的中央杏仁核外侧部、终纹床核外侧部中间区。
Object:To identify the nuclei related to the jaw opening reflex(JOR)which is evoked by the oral facial noxious stimuli with virus-tracing techniques,especially the nuclei in the diencephalon and the limbic system.Methods:Anterograde polysynaptic virus as well as retrograde polysynaptic virus were applied to identify the related nuclei in the jaw movements accomplished by anterior belly of the digastric muscle and pulp sensation.Results:nuclei were marked over the whole brain in the experimental group.12 nuclei marked with both viruses were distributed in telencephalon,diencephalon,limbic system,mesencephalon and reticular structure.Conclusion:Several nuclei including parasubthalamic nucleus(PSTn),paraventricular hypothalamic nucleus,posterior part(PaPo),zona incerta(ZI)in the diencephalon and central amygdaloid nucleus,lateral division(CeL),bed nucleus of the stria terminalis,lateral division,intermediate part(BSTLI)in the limbic system are related to the JOR.Their pathological changes may explain the oral and maxillofacial symptoms in certain cerebral degenerative diseases,and may also be the potential etiology of temporomandibular joint disorder syndrome.
作者
曲陶然
屠逸琳
苏俭生
QU Tao-ran;TU Yi-lin;SU Jian-sheng(Department of Prothodontics,School and Hospital of Stomatology,Tongji University,Shanghai Engineering Research Center of Tooth Regeneration,Shanghai 200072,China)
出处
《口腔颌面外科杂志》
CAS
2018年第5期252-257,共6页
Journal of Oral and Maxillofacial Surgery
基金
国家自然科学基金项目（81671014）
上海市科学技术委员会项目（18441902100）
上海市科学技术委员会项目（17140903600）
关键词
开颌反射
伪狂犬病毒
单纯疱疹病毒
间脑
边缘系统
颞下颌关节紊乱综合征
jaw opening reflex(JOR)
pseudorabies virus
herpes simplex virus
diencephalon
limbic system
disturbance syndrome of temporomandibular joint
