摘要
目的:研究氧化槐果碱(OSC)对离体大鼠胸主动脉环的舒张作用及其机制。方法:取大鼠胸主动脉环(简称“血管环”),以舒张率为指标,以K-H营养液为空白对照,分别考察不同质量浓度的OSC(0.2~1.0 mg/mL)对基础状态的正常血管环,以及经去甲肾上腺素(PE,1×10^-6 mol/L)预收缩的正常或去内皮血管环的舒张作用;分别以一氧化氮合酶抑制剂L-硝基精氨酸甲酯(L-NAME)、环氧合酶抑制剂吲哚美辛(INDO)预孵育大鼠正常胸主动脉环,以4种钾通道阻滞剂[氯化钡(BaCl2)、四乙基胺(TEA)、4-氨基吡啶(4-AP)、格列本脲(Gli)]预孵育去内皮血管环,同法考察不同质量浓度的OSC(0.2~1.0 mg/mL)对上述血管环的舒张作用。结果:与空白对照比较,不同质量浓度的OSC对基础状态的正常血管环的舒张率无显著影响(P>0.05),但0.4~1.0 mg/mL的OSC能显著提高经PE预收缩的正常或去内皮血管环的舒张率(P<0.01),且呈浓度依赖趋势。经L-NAME、INDO、4-AP、BaCl2预孵育后,不同质量浓度的OSC对经PE预收缩的正常或去内皮血管环的舒张率均无显著影响(P>0.05);而经TEA、Gli预孵育后,0.4~1.0 mg/mL的OSC可显著降低经PE预收缩的去内皮血管环的舒张率(P<0.01)。结论:OSC在试验剂量(0.2~1.0 mg/mL)范围内对基础状态的大鼠胸主动脉环无明显舒张作用,但0.4~1.0 mg/mL的OSC对经PE预收缩的正常或去内皮大鼠胸主动脉环均有明显舒张作用;其血管舒张的作用机制为非内皮依赖性,可能与受体操纵性钙通道、钙激活钾通道和ATP敏感钾通道有关。
OBJECTIVE:To study the vasodilatory effect of oxysophocarpine(OSC)on isolated thoracic aortic rings of rats and its possible mechanism.METHODS:Thoracic aortic rings of rats were collected(called“vascular ring”for short).Using K-H nutrient solution as blank control and the diastolic rate as index,the effects of different concentrations(0.2-1.0 mg/mL)of OSC on normal vascular rings in basal state,normal or endothelium-free vascular rings pre-contracted by norepinephrine(PE,1×10-6 mol/L)were investigated.After pre-culturing normal thoracic aortic rings by nitric oxide synthase inhibitor L-nitro-arginine methyl ester(L-NAME)and cyclooxygenase inhibitor indomethacin(INDO),as well as pre-culturing endothelium-free vascular rings by potassium ion channel blocker BaCl2,tetraethylammonium(TEA)and 4-aminopyridine(4-AP),the diastolic effects of OSC of different concentrations(0.2-1.0 mg/mL)on the above vascular rings were investigated by using the same method.RESULTS:Compared with blank control,there was no significant effects of different concentrations of OSC on the diastolic rate of normal vascular rings in basal state(P>0.05),but 0.4-1.0 mg/mL OSC could significantly improve the diastolic rate of normal or endothelium-free vascular rings pre-contracted by PE(P<0.01),in concentration-dependent manner.After preculturing with L-NAME,INDO,4-AP and BaCl2,different concentrations of OSC had no significant effect on the diastolic rate of normal or endothelium-free vascular rings pre-contracted by PE(P>0.05).After pre-culturing with TEA and Gli,0.4-1.0 mg/mL OSC could significantly reduce the diastolic rate of endothelium-free vascular rings pre-contracted by PE(P<0.01).CONCLUSIONS:OSC did not significantly dilate the thoracic aortic rings of rats in the basal state within the dose range(0.2-1.0 mg/mL),but OSC of 0.4-1.0 mg/mL have significant diastolic effects on the normal or endothelium-free thoracic aortic rings of rats pre-contracted with PE.The mechanism of thoracic aortic rings dilation is endothelium-independent,wh
作者
乔海琦
闫琳
余洋
常智
王佳玲
裴延敏
周茹
QIAO Haiqi;YAN Lin;YU Yang;CHANG Zhi;WANG Jialing;PEI Yanmin;ZHOU Ru(School of Pharmacy,Ningxia Medical University,Yinchuan 750004,China;Ningxia Modernization Engineering Technology Research Center for Hui Medicine and Ningxia Collaborative Innovation Center of Ningxia Medical University,Yinchuan 750001,China;Modernization Key Laboratory for Hui Medicine Co-founded by Ningxia Hui Autonomous Region and Ministry of Education,Ningxia Medical University,Yinchuan 750001,China)
出处
《中国药房》
CAS
北大核心
2019年第22期3057-3061,共5页
China Pharmacy
基金
国家自然科学基金资助项目（No.81660674）
2017年宁夏回族自治区科技创新领军人才培养项目（No.KJT2017005）
2017年宁夏医科大学青年骨干人才培育计划（No.宁医校发〔2017〕119号）
关键词
氧化槐果碱
胸主动脉环
血管舒张
内皮依赖性
钾通道
钙通道
机制
Oxysophocarpine
Thoracic aortic rings
Vasodilation
Endothelium independent
Potassium channel
Calcium channel
Mechanism
