摘要
目的 初步探讨Axl高表达对裸鼠胃癌细胞皮下成瘤的影响。方法 通过胃癌手术病理染色分析Axl与胃癌间的关系;通过转染慢病毒构建高表达Axl的胃癌细胞;通过对皮下注射胃癌细胞建立裸鼠胃癌细胞皮下成瘤模型;通过小动物活体成像在体评估皮下肿瘤大小。结果 胃癌手术病人切除组织免疫组化结果显示,Axl在胃癌组织中表达量显著升高;所构建Axl高表达胃癌细胞中Axl表达量升高2. 4倍。利用Axl高表达胃癌细胞建立裸鼠皮下成瘤模型,小动物活体成像显示皮下注射细胞2周后,高表达Axl细胞所形成的皮下瘤明显大于对照组,4周后,差异更加显著(P<0. 01)。最后,高表达Axl胃癌细胞所形成的皮下瘤的体积和重量均显著大于对照组。结论 胃癌组织高表达Axl,Axl高表达能够增强胃癌细胞SGC-7901的皮下成瘤能力。
Objective To evaluate the effect of Axl overexpression on subcutaneous tumorigenesis of gastric cancer cell lines in nude mice.Methods The relationship between Axl and gastric cancer was analyzed by immunohistochemical staining.Axl-overexpressing gastric cancer cells were constructed through lentivirus transfection.The nude mouse model was established by subcutaneous injection of gastric cancer cells,and tumor sizes were evaluated by imaging of live animals.Results Immunohistochemical result revealed significantly higher Axl expression in gastric cancer tissues compared with adjacent tissues.Axl expression in the Axl-overexpression gastric cancer cell line was increased by 2.4-fold.Live imaging of model mice revealed that after subcutaneous injection of cell lines for 2 weeks,tumors constructed with the Axl-overexpression cell line were significantly larger than that of the control group,and this difference was more significant after 4 weeks(P<0.01).Finally,the volume and weight of subcutaneous tumors formed by gastric cancer cells with high Axl expression were significantly increased compared with the control group.Conclusions Axl overexpression can enhance the subcutaneous tumorigenicity of SGC-7901 cells.
作者
田苗苗
田祖宏
张慧霞
张莹
宁思明
聂勇战
胡思隽
TIAN Miaomiao;TIAN Zuhong;ZHANG Huixia;ZHANG Ying;NING Siming;NIE Yongzhan;HU Sijun(State Key Laboratory of Cancer Biology,Xijing Hospital of Digestive Diseases,the Fourth Military Medical University/the First Affiliated Hospital of Air Force Medical University,Xi’an 710032,China)
出处
《中国比较医学杂志》
CAS
北大核心
2019年第11期1-6,共6页
Chinese Journal of Comparative Medicine
基金
国家自然科学基金面上项目(81672702)
空军军医大学西京医院学科助推项目(XJZT18MJ84)
关键词
AXL
胃癌
小动物活体成像
皮下成瘤
裸鼠
Axl
gastric carcinoma
living imaging of small animals
subcutaneous tumorigenesis
nude mouse
