摘要
垂体特异性转录因子(Pou1f1)与动物的生长发育密切相关,本研究运用免疫组化法、荧光定量PCR及Western Blot等方法对Pou1f1在乾华肉用美利奴羊的背最长肌、胸肌和半腱肌的转录和蛋白表达水平进行了检测,研究结果表明:POU1F1蛋白在乾华肉用美利奴羊背最长肌、胸肌、半腱肌中等3个不同肌肉组织的肌纤维细胞质中均有阳性表达;Pou1f1基因在乾华肉用美利奴羊背最长肌、胸肌、半腱肌等3个不同部位的不同肌肉的mRNA和蛋白水平均差异均不显著(P>0.05),但均呈现半腱肌>胸肌>背最长肌的趋势。说明Pou1f1基因可以作为肉羊生长发育特别是肌肉发育的遗传标记。
Pituitary specifific transcription factor l(POU F1)is closely related to the growing development of animals. In this study, immunohistochemistry, qRT-PCR and Western Blot were used to detect the transcription and protein expression levels of the POU1F1 in musculi longissimus thoracis,pectorales and semitendinosus. The research results show that, POU1F1 protein was positive expression in muscle fiber cytoplasm of three different muscle tissues in longissimus dorsi,pectorales and semitendinosus of Qianhua Mutton Merino. POU1F1 gene showed no significant difference in mRNA and protein levels of longissimus dorsi, pectorales, and semitendinosus in three different parts of Qianhua Mutton Merino(P>0.05). But there was a tendency that semitendinosus>pectorales>longissimus dorsi. It explained that POU1F1 could be used as a genetic marker for growing development of mutton sheep.
作者
李佳蓉
贾超
姜怀志
马志华
Li Jiarong;Jia Chao;Jiang Huaizhi;Ma Zhihua(College of Animal Science and Technology,Jilin Agricultural University,Jilin Changchun 130118;Ministry of Education Key Laboratory of Animal Production and Product Quality and Safety,Jilin Agricultural University,Jilin Changchun 130118;Qianan Zhi-hua Breeding Sheep co.LTD,Jilin Qianan 131404)
出处
《现代畜牧兽医》
2019年第11期1-6,共6页
Modern Journal of Animal Husbandry and Veterinary Medicine
基金
吉林省科技发展计划项目（20160204018NY）
关键词
垂体特异性转录因子
肌肉
基因表达
乾华肉用美利奴羊
Pituitary-specific transcription factor
Muscle
Gene expression
Qianhua mutton merino
