摘要
目的:对葡萄球菌性烫伤样皮肤综合征(SSSS)快速诊断试剂盒的研发进行探索。方法:采用表皮剥脱毒素A抗体(Anti-ETA)、表皮剥脱毒素A(ETA),优化反应条件后将Anti-ETA包被ELISA板,初步建立SSSS表皮剥脱毒素A的ELISA检测方法。结果:在建立的ELISA方法中,Anti-ETA的最佳包被浓度为10μg·mL-1,酶标二抗最佳工作浓度为1∶100。对7份SSSS患者血清、7份内毒素阳性患者血清及7份正常人血清进行吸光度值(OD 450 nm)的检测,结果显示SSSS患者血清组和正常人血清组的OD值无差别(P>0.05),即单纯包被Anti-ETA尚不能作为SSSS的有效检测。结论:本试验初步建立以Anti-ETA作为包被抗体检测SSSS表皮剥脱毒素A的ELISA方法,但单纯包被Anti-ETA检测灵敏度低,研制SSSS的快速诊断试剂盒需进一步结合Anti-ETB作为共同包被抗体。
Objective:To explore the development of a rapid diagnostic kit for staphylococcal scalded skin syndrome(SSSS).Methods:Epidermal exfoliation toxin A antibody(Anti-ETA)and Epidermal exfoliation toxin A(ETA)were used.After optimizing the reaction conditions,Anti-ETA was coated on ELISA plate to established an ELISA assay for detecting ETA.Results:In the established ELISA method,the optimum coating concentration of Anti-ETA was 10μg·mL-1,and the optimum working concentration of ELISA was 1∶100.Hypothesis tests were carried on the OD values of 7 SSSS patient sera,7 endotoxin-positive patient sera,and 7 normal human sera.The results showed that there was no difference in OD value between SSSS patients and normal persons(P>0.05),that is to say,anti-ETA alone could not be used as an effective detection of SSSS.Conclusions:An ELISA method for detecting SSSS epidermal exfoliation toxin A using Anti-ETA as coated antibody was preliminarily established in this experiment.However,the sensitivity of detecting SSSS epidermal exfoliation toxin A by anti-ETA alone is low.The development of a rapid diagnostic kit for SSSS requires further combination with Anti-ETB as a common coated antibody.
作者
赖芸
尤聪
李立明
李龙年
叶小英
LAI Yun;YOU Cong;LI Li-ming;LI Long-nian;YE Xiao-ying(Gannan Medical University,Ganzhou,Jiangxi 341000;Gannan Medical University,The First Affiliated Hospital,Ganzhou,Jiangxi 341000)
出处
《赣南医学院学报》
2019年第10期1007-1010,1031,共5页
JOURNAL OF GANNAN MEDICAL UNIVERSITY
基金
江西省科技计划项目(编号:20161BBG70324)
