摘要
目的:观察鸢尾素(Irisin)对肥胖大鼠子宫内膜功能的影响。方法:选用成年雌性SD大鼠,采用高脂饲料建立肥胖模型;正常组给予正常饮食。将造模成功大鼠随机分为肥胖模型组(16只)和Irisin组(16只),另设对照组(16只)。其中,Irisin组腹腔注射重组Irisin[100 ng/(g·d)×14 d],肥胖模型组和对照组腹腔注射生理盐水,并于注射前后记录大鼠体质量。后于动情期内合笼,于大鼠妊娠D5、D10天每组各处死8只大鼠,记录妊娠率及平均胚泡着床数,观察子宫内膜组织形态;检测大鼠子宫内膜白血病抑制因子(LIF)、整合素αvβ3蛋白及mRNA的表达。结果:Irisin组经腹腔注射Irisin 14 d后,大鼠体质量低于肥胖模型组,差异有统计学意义(P=0.035)。肥胖模型组平均胚泡着床数比对照组和Irisin组低,差异有统计学意义(均P<0.05)。对照组大鼠子宫内膜宫腔饱满,胚胎发育好;肥胖模型组大鼠子宫腔间隙较大,胚胎发育不良;Irisin组大鼠子宫腔接近闭合,胚胎发育良好。肥胖模型组子宫内膜LIF、αvβ3的蛋白表达量和mRNA含量比对照组和Irisin组低,差异有统计学意义(均P<0.05)。结论:Irisin可通过增加子宫内膜LIF、αvβ3的表达,从而改善肥胖大鼠子宫内膜容受性。
Objective:To investigate the effect of Irisin on the endometrial function in obese rats.Methods:Female SD rats were treated with high-fat diets to induce obesity, while the normal-control group(n=16)was treated with normal diets. The obese rats were randomly divided into the model-control group(n=16) and the irisin group(n=16). The rats in the irisin group were injected intraperitoneally with recombinant irisin [100 ng/(g·d)×14 days], while the rats in the two control groups were injected with normal saline. Then, female rats in estrus were mated with male rats. Eight rats were sacrificed in each group on the day 5 and day 10 of pregnancy. The pregnancy rate, average number of blastocyst and embryo morphology were observed. The expressions of leukemia inhibitory factor(LIF) and integrin αvβ3 in the implantation sites were determined. Results:The body weight in the irisin group after treatment was significantly lower than that in the model-control group(P =0.035), and the average number of blastocyst in the model-control group were significantly lower than that in the irisin group and the normal-control group(both P<0.05). The endometrium of the normal-control group was full, and the embryo was well developed. The uterine cavity in the model-control group was large, and the embryonic development was poor. The endometrium of the irisin group was closed, and the embryonic development was well improved. The expression levels of LIF and integrin αvβ3 mRNAs and proteins in the model-control group were significantly lower than those in the normal-control group and the irisin group(all P<0.05). Conclusions:Irisin can improve the endometrial receptivity of obese rats by increasing the expressions of LIF and integrin αvβ3 in endometrium.
作者
周丽
李成刚
赵玉娟
陈丽
ZHOU Li;LI Cheng-gang;ZHAO Yu-juan;CHEN Li(Department of Traditional Chinese Medicine,Affiliated Dongfeng Hospital,Hubei University of Medicine,Shiyan 442000,Hubei Province,China;School of Pharmaceutical Sciences,Hubei University of Medicine,Shiyan 442000,Hubei Province,China)
出处
《国际生殖健康／计划生育杂志》
CAS
2019年第6期467-470,530,共4页
Journal of International Reproductive Health/Family Planning
基金
湖北省教育厅科学研究指导性项目（B2018437）
十堰市科学研究引导性项目（18Y08
19Y94）
