摘要
目的探讨RhoE对神经细胞凋亡的作用及可能的相关机制。方法用免疫组织化学染色、Q-PCR和免疫印迹等方法检测RhoE-/-及RhoE+/+小鼠脑组织内RhoE、Caspase3和P65蛋白表达水平。结果 RhoE-/-小鼠脑组织内无RhoE蛋白表达;RhoE-/-小鼠多部位脑组织cleaved-Caspase3表达水平较RhoE+/+小鼠显著降低(P<0.05);同时RhoE-/-小鼠脑组织内P65蛋白表达水平较RhoE+/+小鼠显著增高(P<0.05),但mRNA水平无明显改变(P>0.05)。结论 RhoE在小鼠中枢神经系统内可能通过NF-κB信号通路中P65蛋白表达水平来调控神经元的凋亡;RhoE可能是一个新的神经元凋亡调控位点。
Objective To explore the effect and mechanism of the RhoE on neurocytes apoptosis.Methods Immunohistochemistry,Q-PCR and western blot were performed to explore RhoE,P65 and cleaved-Caspase3’s expression levels in RhoE-/- and RhoE+/+mice brain tissue.Results Immunohistochemistry showed there was no expression of RhoE in RhoE-/- mice brain.Compared with RhoE+/+ mice,cleaved-Caspase3 protein level decreased in RhoE-/- mice’s brain tissue(P<0.05).On the other hand,P65 protein level increased in RhoE-/- mice’s brain(P<0.05),but there’s no significant difference in the mRNA level(P>0.05).Conclusion The results showed the RhoE could regulated neurocytes apoptosis through P65 expression level in central nervous system.The RhoE probably regulated P65 in protein level.And the RhoE could be a new regulatory site for neurocytes apoptosis.
作者
董慧敏
吕龙琴
董若辰
刘宝辉
毛善平
Dong Huimin;Lv Longqin;Dong Ruochen(Department of Neurology,Renmin Hospital of Wuhan University,Wuhan 430060)
出处
《卒中与神经疾病》
2019年第5期509-512,530,共5页
Stroke and Nervous Diseases
基金
国家自然科学基金资助项目(编号为81502175,81371390)
