摘要
目的研究癌胚抗原(CEA)、神经元特异性烯醇化酶(NSE)、鳞状上皮癌细胞抗原(SCCA)联合miR 21检测与肺癌患者临床病理特征、疗效及预后的相关性。方法选择87例肺癌患者作为实验组和同期体检的健康人作为对照组,采用电化学发光免疫法检测各组患者血清中CEA、NSE、SCCA的表达,实时定量PCR检测血清中miR 21的表达,治疗前分析肿瘤标志物CEA、NSE、SCCA及miR 21与肺癌患者中的性别、年龄、病理类型及TNM分期临床病理特征的相关性,治疗后分析与疗效及预后的相关性。结果①与对照组相比,实验组患者CEA、NSE、SCCA及miR 21的表达水平显著升高,差异有统计学意义(P<0.05);②CEA、SCCA、miR 21的表达水平与肺癌患者临床病理特征无关,差异无统计学意义(P>0.05),NSE与肺癌的TNM分期相关,差异有统计学意义(P<0.05);③CEA、NSE、SCCA及miR 21的表达水平与疗效相关,差异有统计学意义(P<0.05);④生存曲线显示,CEA、NSE、SCCA及miR 21的表达水平与患者生存预后相关,差异有统计学意义(P<0.05);⑤4种指标联合诊断肺癌的灵敏度显著优于单项指标,差异有统计学意义(P<0.05)。结论肺癌患者血清中CEA、NSE、SCCA和miR 21的表达水平显著高于正常人,可作为肺癌的诊断、疗效及预后的指标。
Objective To investigate the relationship between CEA,NSE,SCCA combined with miR-21 and clinicopathological features, curative effect and prognosis in patients with lung cancer.Methods 87 patients with lung cancer were selected as the experimental group and 87 healthy people in the same period of physical examination as the control group. The expression levels of CEA,NSE and SCCA in serum were detected by electrochemiluminescence immunoassay,and miR-21 was detected by real-time PCR.The correlation of tumor markers CEA,NSE,SCCA and miR-21 with sex,age,pathological type and clinicopathological characteristics of TNM stage in patients with lung cancer was analyzed before treatment,and the correlation with curative effect and prognosis was analyzed after treatment. Results ①compared with the control group,the expression levels of CEA,NSE,SCCA and miR-21 in the experimental group were significantly higher than those in the control group(P<0.05).②The expression levels of CEA,SCCA and miR-21 were not correlated with clinicopathological features of lung cancer(P>0.05). NSE was correlated with TNM stage of lung cancer(P<0.05).③The expression levels of CEA,NSE,SCCA and miR-21 were correlated with the curative effect(P<0.05).④The survival curve showed that the expression levels of CEA,NSE,SCCA and miR-21 were correlated with the survival prognosis of the patients(P<0.05).⑤The sensitivity of the combination of the four indexes in the diagnosis of lung cancer were significantly better than those of single index,and the difference was statistically significant(P<0.05). Conclusion the expression levels of CEA,NSE and SCCA in serum of patients with lung cancer are significantly higher than those of normal controls,and the expression level of miR-21 is significantly lower than that of normal controls,which could be used as indicators of diagnosis,curative effect and prognosis of lung cancer.
作者
汤乔雅
宫郡茗
马艳凌
TANG Qiaoya;GONG Junming;MA Yanling(Tianjin Medical University,Tianjin,China,300070;Department of Oncology,Third People’s Hospital of Hubei Province,Wuhan,Hubei,China,430033)
出处
《分子诊断与治疗杂志》
2019年第6期508-512,共5页
Journal of Molecular Diagnostics and Therapy
基金
天津市滨海新区卫生计生委科技项目(2015BWKY002)
