摘要
农作物秸秆转化为氢气的一个瓶颈问题是制氢菌种产氢性能低下.为解决这一问题,以农作物秸秆水解液的主要成分木糖为碳源,从羊粪中分离获得一株以木糖为碳源产氢性能高的暗细菌.该细菌经16S rRNA鉴定菌属后,发现与Escherichia coli相似度高达99%,将其命名为Escherichia coli XRN510.此后对该暗细菌的碳源、氮源利用情况、产氢液初始pH、木糖浓度、氮源种类、氮源比例等参数进行了优化研究,获得Escherichia coli XRN510的最优产氢条件.最优产氢条件为:木糖为碳源,木糖浓度为10.0 g/L,L-谷氨酸和酵母膏的组合为氮源,且L-谷氨酸和酵母膏的添加比例为3∶5,产氢液初始pH为7.2.在最优的条件下,暗细菌Escherichia coli XRN510的产氢量为1468.93±61.52 mL/L.研究获得一株可高效发酵木糖制氢的暗细菌及其最优制氢条件,可为农作物秸秆水解液为底物大规模制氢提供菌种资源及工艺条件.
The low efficiency hydrogen-producing bacteria is one of the major bottlenecks for the conversion of crop straw into hydrogen.To solve this problem,an high efficiency hydrogen-producing bacteria using xylose as carbon source was isolated from sheep manure and identified as Escherichia coli XRN510 by 16S rRNA gene sequence analysis.Thereafter,the effects of carbon source,nitrogen source,initial pH,xylose concentration,the types of nitrogen sources and the ratio of different nitrogen sources were studied and the optimum conditions for hydrogen production were obtained.The optimum hydrogen production conditions were a xylose concentration of 10.0 g/L,nitrogen source of L-glutamic acid and yeast extract,a L-glutamic acid to yeast extract ratio of 3∶5,and an initial pH of 7.2.This resulted in a maximum hydrogen yield of 1468.93±61.52 mL/L.A high efficiency bacterium capable of fermenting xylose to produce hydrogen and its optimal hydrogen production conditions were obtained in this study,which could provide strain resources and technological conditions for large-scale hydrogen production from crop straw hydrolysate.
作者
安丹
徐瑞娜
谢林花
陆遥
AN Dan;XU Rui-na;XIE Lin-hua;LU Yao(School of Environmental Science and Engineering,Shaanxi University of Science&Technology,Xi′an 710021,China)
出处
《陕西科技大学学报》
CAS
2019年第6期24-32,共9页
Journal of Shaanxi University of Science & Technology
基金
陕西省科技厅自然科学基础研究计划项目(2018JQ5216)
陕西科技大学博士科研启动基金项目(2016BJ-08)
