摘要
邻苯二甲酸二甲酯(DMP)是一种使用面广、年产量大的人工合成有机化合物,扩散到周围环境中会造成环境污染,去除DMP的主要方法为生物降解法,环境因素是影响生物修复的重要因素。以DMP降解菌Paracoccus sp.QD15-1为研究对象,利用紫外分光光度计、高效液相色谱和反转录-聚合酶链式扩增(RT-PCR)技术,研究了在基础无机盐液体培养基中外加碳氮源以及不同pH和温度对菌株生物量、DMP降解率和降解基因表达的影响。结果发现:在不同碳源的生长环境中,加入质量浓度1.0 g/L乳糖后,Paracoccus sp.QD15-1的生物量最大,DMP降解率达到42.16%,基因phtAb和phtB的表达量最大;在不同氮源的生长环境中,加入质量浓度1.0 g/L硝酸铵后菌株的生物量最大,降解能力最强,基因phtAb和phtB的表达量均最多;在不同pH的生长环境中,pH=8时菌株生物量最多且DMP降解能力最好;在不同温度的生长环境中,当温度为25℃时菌株的生物量最大,DMP降解率为37.80%。pH=8,温度为25℃时,基因phtAb和phtB的表达量均最多。因此,Paracoccus sp.QD15-1在不同环境条件下生长能力和降解DMP能力均较强,能适应不同的生长环境,在DMP污染的生物修复工程实践中具有良好的利用前景。
Dimethyl phthalate(DMP)was a synthetic organic compounded with a widespread application and a large annual production.It spreaded in the surrounded environment,and caused environmental contamination.Biodegradation was the main method to eliminate DMP,and the environmental factors were vital factors to affect bioremediation.DMP degradation Paracoccus sp.QD15-1 was selected as the research object,and the UV source spectrophotometer,high performance liquid phase and RT-PCR were applied to study the biomass,DMP degradation rate and the degradation gene expression at different carbon sources,nitrogen sources,pH and temperature in the basic inorganic salt medium.The results showed that in the different carbon sources,1.0 g/L lactose got the maximum strain biomass and the DMP degradation rate reached 42.16%.In addition,the expression of degradation gene phtAb and phtB were the largest.In the different nitrogen sources,the 1.0 g/L ammonium nitrate got the maximum strain biomass and the strongest degradation ability.In addition,the expression of degradation genes phtAb and phtB were the highest.Of the different pH,pH=8 got the maximum strain biomass and the best degradation ability.Of the different temperature,25℃ got the maximum strain biomass and the best degradation rate which was 37.80%.When pH=8 and temperature was 25℃,the expression of degradation genes phtAb and phtB were the highest.Therefore,Paracoccus sp.QD15-1 had strong growth ability and DMP degradation ability under different environmental conditions,and could adapt to different growth environments,and had a good utilization prospect in DMP-contaminated bioremediation engineering practice.
作者
刘虹
朱晓慧
郭茹鑫
王恒煦
王志刚
LIU Hong;ZHU Xiaohui;GUO Ruxin;WANG Hengxu;WANG Zhigang(College of Life Sciences and Agriculture Forestry,Qiqihar University,Qiqihar Heilongjiang 161006)
出处
《环境污染与防治》
CAS
CSCD
北大核心
2019年第11期1319-1324,共6页
Environmental Pollution & Control
基金
国家自然科学基金资助项目(No.31670375、No.31870493)
齐齐哈尔市科学技术计划项目(No.NYGG-201616)
关键词
邻苯二甲酸二甲酯
碳源
氮源
PH
温度
降解基因
dimethyl phthalate
carbon source
nitrogen source
pH
temperature
degradation gene
