摘要
以冷藏草鱼的优势腐败菌气单胞菌(Aeromonas spp.)为研究对象,利用报告菌株Chromobacterium violaceum CV026检测细菌的群体感应活性,通过染色法定量测定细菌的生物膜形成,并探究温度以及群体感应信号分子对气单胞菌生物膜形成的影响。结果显示,冷藏草鱼的气单胞菌属包含多个不同种,分离获得的20株气单胞菌中90%能产生N-酰基高丝氨酸内酯。进一步分析生物膜形成量较强的2株菌A.salmonia W41和A.salmonia W69,结果显示该2株菌在30℃和8℃的生物膜形成量高于37℃。菌株W41和W69主要分泌N-丁酰基高丝氨酸内酯(N-butanoyl-L-homoserine lactone,C4-HSL)和N-己酰基高丝氨酸内酯(N-hexanoyl-L-homoserine lactone,C6-HSL)信号分子,50μmol/L的C6-HSL显著促进W41生物膜的形成;而低浓度的C4-HSL和C6-HSL促进W69生物膜的形成,高浓度的C4-HSL和C6-HSL抑制W69生物膜的形成。可以看出,大多数气单胞菌属具有群体感应现象,气单胞菌属生物膜的形成受个体差异和温度的影响,同时群体感应信号分子的浓度和类型也是影响气单胞菌属生物膜形成的主要因素。
This study aimed to investigate the quorum sensing(QS)activity and biofilm formation of Aeromonas spp.isolated from refrigerated grass carp as the dominant spoilage organism.The QS activity was detected using the reporter strain Chromobacterium violaceum CV026,and the biofilm formation was quantified by staining method.Then the effect of temperature and QS signaling molecules on the biofilm formation was explored.The results showed that the isolated Aeromonas spp.contained a plurality of species,and 90%of the 20 isolates could secret acyl-homoserine lactones(AHLs).Two strains of A.salmonia W41 and A.salmonia W69 with strong biofilm formation ability were chosen for further analysis.The results indicated that the biofilm formation ability of the two strains was significantly higher at 30℃and 8℃than at 37℃.Both strains mainly produced N-butanoyl-L-homoserine lactone(C4-HSL)and N-hexanoyl-L-homoserine lactone(C6-HSL)signaling molecules.The biofilm formation of W41 was significantly promoted by 50μmol/L C6-HSL.Low concentrations of C4-HSL and C6-HSL promoted the biofilm formation of W69,but high concentrations of C4-HSL and C6-HSL had an inhibitory effect.The present study exhibited that most Aeromonas spp.showed QS phenomenon,and the biofilm formation of Aeromonas spp.was affected not only by individual differences and temperature,but also by the concentration and type of QS signaling molecules.
作者
张彩丽
王辰晨
朱菲菲
刘海梅
刘岩龙
ZHANG Caili;WANG Chenchen;ZHU Feifei;LIU Haimei;LIU Yanlong(School of Food Engineering,Ludong University,Yantai 264025,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2019年第22期155-160,共6页
Food Science
基金
山东省自然科学基金项目(ZR2018BC060)
鲁东大学引进人才科研项目(LB2017016)
山东省重点研发计划(医用食品)项目(2018YYSP009)
关键词
草鱼
气单胞菌属
群体感应
生物膜
Ctenopharyngodon idellus
Aeromonas spp.
quorum sensing
biofilm
