摘要
黑脉羊肚菌多糖(polysaccharides from Morchella angusticeps Peck,PMEP)已被证实可以降低胆固醇。本研究旨在比较PMEP及其硫酸化多糖(sulfated PMEP,SPMEP)对高胆固醇饮食大鼠的降胆固醇活性。结果表明:高剂量的PMEP和SPMEP可使大鼠的血清总胆固醇质量浓度分别降低15%和23%,表明SPMEP比PMEP具有更强的降胆固醇活性。PMEP和SPMEP降低血清胆固醇质量浓度伴随着肝脏3-羟基-3-甲基戊二酰CoA还原酶表达显著下调及7α-羟化酶表达显著上调(P<0.05)。此外,PMEP和SPMEP都可以显著降低肝脏胆固醇质量浓度(P<0.05),同时显著提高粪便和肠道胆汁酸的总量(P<0.05)。结论:PMEP通过硫酸化修饰可以增强对大鼠的降胆固醇活性。
Polysaccharide extracted from Morchella angusticeps Peck(PMEP) has been proved to have a hypocholesterolemic effect. This study aimed to compare the cholesterol-lowering activity of PMEP and its sulfated derivative(SPMEP) in rats fed on a high-cholesterol diet. Results showed that PMEP at high dose and SPMEP could decrease serum total cholesterol by 15% and 23%, respectively, indicating that SPMEP had a stronger cholesterol-lowering potency than PMEP(P<0.05). PMEP and SPMEP down-regulated the expression of hepatic 3-hydroxy-3-methyl-glutarylcoenzyme A reductase and up-regulated the expression of cholesterol-7α-hydroxylase(P<0.05). Besides, both of them could decrease liver cholesterol, while significantly increasing the excretion of total fecal and intestinal bile acids(P<0.05). Therefore, the cholesterol-lowering activity of PMEP in rats can be enhanced after being sulfated.
作者
唐瑜婉
张月巧
李瑶
雷琳
李富华
赵吉春
吴素蕊
明建
TANG Yuwan;ZHANG Yueqiao;LI Yao;LEI Lin;LI Fuhua;ZHAO Jichun;WU Surui;MING Jian(College of Food Science,Southwest University,Chongqing 400715,China;Research Center of Food Storage&Logistics,Southwest University,Chongqing 400715,China;Kunming Edible Fungi Institute,All China Federation of Supply and Marketing Cooperatives,Kunming 650223,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2019年第21期136-142,共7页
Food Science
基金
国家自然科学基金面上项目(31271825
31471576)
关键词
黑脉羊肚菌
多糖
硫酸化修饰
胆固醇代谢
Morchella angusticepes Peck
polysaccharide
sulfation
cholesterol metabolism
