摘要
小球藻胞内多糖等活性物质具有多种重要生物活性和生理功能。为提高小球藻EC04胞内多糖的产率,采用单因素实验和L9(34)正交实验优化对其培养条件进行优化。在暗光比12∶12、温度24±1℃和2000Lux光照条件下,EC04产胞内多糖的最佳条件为:MgSO4·7H2O浓度112.5mg·L^–1,K2HPO4浓度60mg·L^–1,NaNO3浓度1.2g·L^–1,维生素B1浓度0.5mg·L^–1,维生素B12浓度0.1μg·L^–1。在此条件下,EC04产糖率为271.74mg·g^–1,细胞密度为48.027×10^6cells·mL^–1,与基础培养基相比分别提高了97.49%和34.91%。添加VB1、VB12等也在不同程度上影响小球藻胞内多糖产率。对所提取胞内多糖进行抗氧化活性初探,结果表明其具有一定的自由基的清除能力,对DPPH·(1-二苯基-2-三硝基苯肼)和 OH(羟自由基)的最大清除率分别达到了82.32%和64.27%。
Polysaccharides in Chlorella are involved in many important biological activities and physiological functions.To improve the intracellular polysaccharide production of Chlorella,culture conditions were investigated by single factor experiment and orthogonal experiment.The results showed that the maximum polysaccharide accumulation was obtained at MgSO4·7H2O 112.5 mg·L^–1,K2HPO460 mg·L^–1,NaNO31.2 g·L^–1,VB10.5 mg·L^–1,and VB120.1μg·L^–1.Under these conditions,the polysaccharide production and biomass were 271.74 mg·g^–1 and 48.027×10^6 cells·mL^–1,which increased by 97.49%and 34.91%,respectively.Free radical scavenging rate of the polysaccharide was also studied.The results showed maximum scavenging rates of DPPH radical and hydroxyl radical were 82.32%and 64.27%,respectively.
作者
杨海燕
李洁琼
刘红全
黄小燕
许钟涛
罗远康
禤金彩
龙寒
YANG Haiyan;LI Jieqiong;LIU Hongquan;HUANG Xiaoyan;XU Zhongtao;LUO Yuankang;XUAN Jincai;LONG Han(Guangxi Colleges and Universities Key Laboratory of Utilization of Microbial and Botanical Resources,School of Marine Sciences and Biotechnology,Guangxi University for Nationalities,Nanning 530008,China;Guangxi Key Laboratory for Polysaccharide Materials and Modifications,School of Marine Sciences and Biotechnology,Guangxi University for Nationalities,Nanning 530008,China)
出处
《热带海洋学报》
CAS
CSCD
北大核心
2019年第6期98-104,共7页
Journal of Tropical Oceanography
基金
广西自然科学基金项目(2018GXNSFAA294032)
广西民族大学研究生教育创新计划项目(Gxun-chxzs 2017117)
广西民族大学大学生创新创业项目(Gxun-chxzs 2017106080)
广西科技基地和人才专项(桂科AD18126005)~~
关键词
小球藻
多糖
营养盐
维生素
自由基
Chlorella sp.
polysaccharide
nutrient
vitamin
radical
