摘要
采用土培的方式,以颠茄(Atropa belladonna)实生苗为试验材料,外源茉莉酸甲酯(MeJA)和水杨酸(SA)作为调控物质,研究外源MeJA和SA单独和复合施用对100 mmol·L-1 NaCl胁迫下颠茄幼苗生理特性、氮代谢及次生代谢的影响。结果表明:与盐胁迫处理相比, MeJA、SA和MeJA+SA复合处理后,颠茄叶片光合色素含量增多,最大荧光(Fm)、最大光化学效率(Fv/Fm)、实际光化学量子产量(Y)、光合电子传递速率(ETR)、光化学淬灭系数(qP)显著提高,初始荧光(F0)、非光化学淬灭系数(NPQ)显著降低,脯氨酸、可溶性糖含量显著提高,抗氧化酶(超氧化物歧化酶、过氧化物酶、过氧化氢酶)活性明显增强,丙二醛含量显著降低,硝态氮、可溶性蛋白、游离氨基酸含量和氮代谢关键酶(硝酸还原酶、谷氨酰胺合成酶、谷氨酸脱氢酶)的活性均显著升高,次生代谢产物莨菪碱和东莨菪碱含量显著提升;且以MeJA+SA复合处理对缓解盐胁迫对颠茄造成的伤害效果最好,优于MeJA和SA单独处理。由此说明MeJA+SA复合处理更能提高颠茄幼苗对盐胁迫抗性, MeJA与SA在缓解颠茄幼苗盐胁迫中表现出积极的协同作用。
In this study, the seedlings of Atropa belladonna were planted by soil culture and treated with exogenous methyl jasmonate(Me JA) and salicylic acid(SA). By investigating the effects of Me JA and SA alone and in combination on physiological characteristics, nitrogen metabolism and secondary metabolism under 100 mmol·L-1 Na Cl stress, we found that compared to Na Cl stress treatment, the photosynthetic pigment contents, maximal fluorescence(Fm), maximal photochemical efficiency(Fv/Fm), actual photochemical quantum yield(Y), photosynthetic electron transport rate(ETR) and photochemical quenching coefficient(qP) greatly increased, but initial fluorescence(F0) and non-photochemical quenching coefficient(NPQ) significantly decreased under Me JA, SA and Me JA+SA treatments. Moreover, proline content, soluble sugar content, and antioxidant enzyme activities dramatically enhanced, while malondialdehyde content reduced significantly. The contents of nitrate nitrogen, soluble protein, free amino acid, and the activities of nitrogen metabolism key enzymes(nitrate reductase, glutamine synthetase and glutamate dehydrogenase) significantly increased. The secondary metabolites, scopolamine and hyoscyamine, were increased significantly. The Me JA+SA treatment was the best to alleviate the damage caused by Na Cl stress on A. belladonna, better than Me JA and SA alone. The result indicates that Me JA+SA treatment could improve the resistance of A. belladonna seedlings to Na Cl stress. Me JA and SA showed positive synergistic effect in relieving Na Cl stress of A. belladonna seedlings.
作者
山雨思
代欢欢
何潇
辛正琦
吴能表
SHAN Yu-Si;DAI Huan-Huan;HE Xiao;XIN Zheng-Qi;WU Neng-Biao(School of Life Science,Southwest University,Chongqing 400715,China;Key Laboratory of Eco-euvironments in Three Gorges Region,Ministry of Education,Chongqing 400715,China)
出处
《植物生理学报》
CAS
CSCD
北大核心
2019年第9期1335-1346,共12页
Plant Physiology Journal
基金
国家自然科学基金(30500041)~~
关键词
颠茄
盐胁迫
茉莉酸甲酯
水杨酸
次生代谢
Atropa belladonna
NaCl stress
methyl jasmonate
salicylic acid
secondary metabolism
