摘要
选取相同养殖条件的10月、22月和34月龄的黄颡鱼和新品种杂交黄颡鱼(黄颡鱼P.fulvidraco♀×瓦氏黄颡鱼P.vachelli♂)“黄优1号”进行形态及性腺发育的比较研究。通过形体指标测量发现“黄优1号”生长性能显著优于黄颡鱼;在22月和34月龄黄颡鱼中,雄性的体重是雌性的2倍左右,雄性生长速度显著高于雌性;而在“黄优1号”中,两性生长异形现象被显著减弱。基于性腺解剖形态分析发现雌性“黄优1号”卵巢完全退化,呈细线状结构且没有卵子产生,故“黄优1号”雌鱼完全不育;雄性“黄优1号”精巢组织呈现透明状和退化状态。精巢组织切片HE染色分析发现10月龄“黄优1号”的精小囊为空腔状几乎没有精子产生,22月和34月龄“黄优1号”的精小囊内出现极少量精子。计算机辅助精子分析系统(CASA)分析发现,相比于黄颡鱼,“黄优1号”精巢中精子量非常少,有效活力低下;经过繁殖能力测试,22月龄“黄优1号”雄鱼不具备繁殖能力。新品种杂交黄颡鱼“黄优1号”在生长性能提高上显现了杂交优势,具有推动黄颡鱼产业发展的潜力。
The yield of hybrid yellow catfish(Pelteobagrus fulvidraco♀×Pelteobaggrus vachelli♂)was gradually increased,but the basic research is rarely.In this study,a comparative analysis of morphology and gonad development of yellow catfish(Pelteobagrus fulvidraco)and hybrid yellow catfish“Huangyou No.1”at 3 various ages was conducted.The hybrid yellow catfish has a growth advantage to yellow catfish.22-month and 34-month-old yellow male catfish weight twice as much as females.However,sexual size dimorphism was greatly reduced in the hybrid yellow catfish.Completely degenerated ovarian with a fine line structure and no egg production were observed in female hybrid yellow catfish,indicating that the female hybrid yellow catfish is completely sterile.Testes of male hybrid yellow catfish were transparent and degenerated.The spermatogenic cyst was almost empty and had no sperm production in 10-month-old male hybrid yellow catfish,while a few sperms were observed in the spermatogenic cyst of 22-month-and 34-month-old male hybrid yellow catfish.Compared to yellow catfish,the number of sperms of hybrid yellow catfish were very small and their activities were low based on computer assisted spermatozoa analysis system(CASA).In addition,22-month-old male hybrid yellow catfish has no reproductive capacity.The novel variety“Huangyou No.1”has a hybrid advantage in growth performance and is expected to promote the development of yellow catfish industry.
作者
胡伟华
丹成
郭稳杰
樊启学
梅洁
HU Wei-Hua;DAN Cheng;GUO Wen-Jie;FAN Qi-Xue;MEI Jie(College of Fisheries,Huazhong Agricultural University,Wuhan 430070,China)
出处
《水生生物学报》
CAS
CSCD
北大核心
2019年第6期1231-1238,共8页
Acta Hydrobiologica Sinica
基金
国家现代农业产业技术体系特色淡水鱼体系项目(CARS-46)资助~~
