摘要
以采自四川石渠的黄绿卷毛菇Floccularialuteovirens为研究对象,采用水提醇沉的经典方法以及单因素试验,并利用Box-BenhnkenDesign(BBD)中心组合试验设计原理,以提取温度、时间以及料液比3个影响因素作为自变量,多糖提取率为考察指标,设计3因素3水平的响应面试验,优化黄绿卷毛菇子实体多糖(FLPs)的提取工艺,并通过·OH和O2-·自由基清除能力考察FLPs的抗氧化能力。最终确定最优水提工艺为提取温度89.31℃,提取时间5.08h,料液比1:48.54(g/mL),且FLPs具有良好的·OH和O2-·自由基清除能力,具有较强的体外抗氧化活性。同时,验证试验证明了该设计方法和模型的准确性和可行性,且在该工艺条件下,多糖提取率有所提高,可为其多糖功能性食品和药品的开发提供理论依据。
The hot-water extraction technique of fruiting body polysaccharides of Floccularia luteovirens(FLPs)collected in Serxü of northwestern Sichuan, Qinghai-Tibet Plateau was optimized by using single factor experimental design and response surface methodology(RSM). Hydroxyl radical and superoxide anion radical scavenging activity were examined to confirm the antioxidant activity of FLPs. The results showed that the optimal hot-water extraction conditions were extraction temperature of 89.31°C, extraction time of 5.08 h, and solid-liquid ratio of 1:48.54 g/mL. Under such a optimization condition, FLPs exhibited strong antioxidant activity of scavenging hydroxyl radical in vitro. It was indicated that the design was reliable and feasible, and could applied to production of functional polysaccharide foods and drugs.
作者
王欢
陈长宝
张波
佟希丹
王淑敏
李玉
WANG Huan;CHEN Chang-Bao;ZHANG Bo;TONG Xi-Dan;WANG Shu-Min;LI Yu(Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi,Jilin Agricultural University,Changchun,Jilin 130118,China;Jilin Provincial Key Laboratory of Ginseng Chemistry and Pharmacology,Jilin Ginseng Academy,Changchun University of Chinese Medicine,Changchun,Jilin 130117,China;Northeast Normal University,Changchun,Jilin 130024,China)
出处
《菌物学报》
CAS
CSCD
北大核心
2019年第10期1681-1688,共8页
Mycosystema
基金
高等学校学科创新引智计划“111”合作基地（D17014）
农业部公益性行业科研专项(201503137)~~
关键词
黄绿卷毛菇
真菌多糖
响应面法
提取工艺
自由基清除
Floccularia luteovirens
fungal polysaccharides
Box-Benhnken Design
extraction condition
radical scavenging
