摘要
[Objectives]To study the antioxidant activity of different solvent extracts of Gynura segetum in vitro,so as to provide experimental basis for further study and utilization of medicinal resources of G.segetum.[Methods]The antioxidant activity of different solvent extracts of G.segetum was determined by UV-vis spectrophotometer using DPPH free radical scavenging method and ABTS^+free radical scavenging method.[Results]The scavenging rates of DPPH and ABTS^+by acetone extract were high,reaching 98.9%and 98.3%,respectively;the scavenging rate of DPPH and ABTS^+by water extract was the lowest,and the scavenging rates were 76.7%and 77.5%,respectively.The order of ABTS^+radical scavenging rate of samples extracted with different extractants was as follows:S 5(acetone extract)>S 1(ethyl acetate extract)>S 6(ethanol extract)>S 4(n-butanol extract)>S 3(methanol extract)>S 2(distilled water extract);the order of DPPH radical scavenging rate of samples extracted with different extractants was as follows:S 5(acetone extract)>S 1(ethyl acetate extract)>S 4(n-butanol extract)>S 6(ethanol extract)>S 3(methanol extract)>S 2(distilled water extract).[Conclusions]The extracts of acetone,ethyl acetate,n-butanol,anhydrous ethanol,methanol and distilled water from the roots of G.segetum had obvious scavenging effects on DPPH and ABTS^+,all had antioxidant components,and acetone had the highest antioxidant effect as an extractant.
[Objectives] To study the antioxidant activity of different solvent extracts of Gynura segetum in vitro, so as to provide experimental basis for further study and utilization of medicinal resources of G. segetum. [Methods] The antioxidant activity of different solvent extracts of G. segetum was determined by UV-vis spectrophotometer using DPPH free radical scavenging method and ABTS+ free radical scavenging method. [Results] The scavenging rates of DPPH and ABTS+ by acetone extract were high, reaching 98.9% and 98.3%, respectively; the scavenging rate of DPPH and ABTS+ by water extract was the lowest, and the scavenging rates were 76.7% and 77.5%, respectively. The order of ABTS+ radical scavenging rate of samples extracted with different extractants was as follows: S5（acetone extract）>S1（ethyl acetate extract）>S6（ethanol extract）>S4（n-butanol extract）>S3（methanol extract）>S2（distilled water extract）; the order of DPPH radical scavenging rate of samples extracted with different extractants was as follows: S5（acetone extract）>S1（ethyl acetate extract）>S4（n-butanol extract） >S6（ethanol extract）>S3（methanol extract）>S2（distilled water extract）. [Conclusions] The extracts of acetone, ethyl acetate, n-butanol, anhydrous ethanol, methanol and distilled water from the roots of G. segetum had obvious scavenging effects on DPPH and ABTS+, all had antioxidant components, and acetone had the highest antioxidant effect as an extractant.
