摘要
考察Fe(Ⅱ)浓度对厌氧氨氧化过程的影响,并采用三维荧光光谱结合平行因子分析方法,解析厌氧氨氧化反应器出水中的荧光组分,探究外加Fe(Ⅱ)与反应器出水水质的关系。结果表明:随着Fe(Ⅱ)浓度从1.84 mg L升至5.00 mg L,NH+4-N和NO-2-N的去除率逐渐增加,表明增加进水Fe(Ⅱ)浓度可以提高微生物对底物的利用率;随着Fe(Ⅱ)浓度的增加,厌氧氨氧化菌的数量亦显著增加;厌氧氨氧化反应器出水的主要荧光组分是类蛋白质和类富里酸物质,随着Fe(Ⅱ)浓度的增加,反应器出水的类蛋白质荧光强度显著增强,表明在一定条件下,投加Fe(Ⅱ)可以促进厌氧氨氧化菌的生长。因此,利用三维荧光光谱法可以反映投加Fe(Ⅱ)对厌氧氨氧化性能的影响,进而反映反应器实际运行状况。
The effect of ferrous ions dosage on the anaerobic ammonia was investigated.The excitation-emission matrix(EEM)fluorescence spectroscopy combined with parallel factor analysis(PARAFAC)method was used to decompose the EEM fluorescence components in the effluent samples.The relationship between the added Fe(Ⅱ)and the effluent quality of the reactor was explored.The results showed that with the increase of ferrous ions concentration from 1.84 mg L to 5.00 mg L,the removal rates of NH+4-N and NO-2-N increased gradually,indicating that increasing the influent ferrous ions concentration could increase the utilization rate of substrate by microorganisms.The proportion of anammox bacteria increased significantly with the increase of ferrous ions dosage;protein-like substances and fulvic acid-like substances were the main fluorophores in the effluent of anammox reactor.With the increase of ferrous ions dosage,the protein-like fluorescence of the effluents increased significantly,which implicated that the dosage of ferrous ions would promote the growth of anammox bacteria under certain conditions.Therefore,EEM fluorescence spectroscopy could reflect the effect of ferrous ions on the performance of anaerobic ammonia oxidation,which then implicate the actual operation of the reactor.
作者
王嵘
姚亮
申慧彦
余丽
李卫华
WANG Rong;YAO Liang;SHEN Huiyan;YU Li;LI Weihua(School of Environmental and Energy Engineering,Anhui Jianzhu University,Hefei 230022,China;Key Laboratory of Water Pollution Control and Wastewater Reuse of Anhui Province,Hefei 230022,China)
出处
《环境工程技术学报》
CAS
2019年第6期658-665,共8页
Journal of Environmental Engineering Technology
基金
国家自然科学基金项目(41601203,51978003)
安徽省重点研发计划项目(1704a0902006)
安徽省自然科学基金项目(1908085ME141,KJ2019JD09)
关键词
厌氧氨氧化
荧光光谱
平行因子分析
Fe(Ⅱ)
类蛋白质
anammox
fluorescence spectra
parallel factor analysis(PARAFAC)
Fe(Ⅱ)
protein-like substance
