摘要
目的探讨丙泊酚麻醉对新生鼠海马区B淋巴细胞瘤-2蛋白(B-cell lymphoma-2,Bcl-2)及其相关蛋白(Bcl-2 associated protein,Bax)表达的影响.方法新生7 d SD大鼠112只,按随机数字表法分为8组(每组14只):对照组1(Con1组),腹腔连续注射5 d生理盐水;P50组、P100组、P150组,分别连续5 d腹腔注射50、100 mg/kg和150 mg/kg丙泊酚;对照组2(Con2组),腹腔连续注射5 d生理盐水,最后1 d在丙泊酚麻醉下行阑尾切除术;SP50组、SP100组和SP150组,分别连续5 d腹腔注射50、100 mg/kg和150 mg/kg丙泊酚,最后1 d在丙泊酚麻醉下行阑尾切除术.实验结束饲养2个月后,取大鼠脑第5段的海马结构,免疫组化法测定海马Bcl-2(抑制凋亡蛋白)、Bax(促凋亡蛋白)的表达.结果与Con1组比较,P50组、P100组、P150组Bax表达逐渐升高(P<0.05),Bcl-2表达逐渐降低,P100组、P150组差异有统计学意义(P<0.05);与P50组比较,P100组、P150组Bax表达升高(P<0.05),Bcl-2表达降低(P<0.05);与Con2组比较,SP50组、SP100组、SP150组Bax表达逐渐升高(P<0.05),SP50组、SP150组Bcl-2表达逐渐降低(P<0.05);与SP50组比较,SP100组、SP150组Bax表达升高(P<0.05),SP150组Bcl-2表达降低(P<0.05).结论反复丙泊酚麻醉通过抑制Bcl-2的活化,增强Bax的表达,可促进新生大鼠海马神经元凋亡,且呈剂量相关性.
Objective To investigate the effects of propofol on the expression of B-cell lymphoma-2 assaciated protein(Bax)and B-cell lymphoma-2(Bcl-2)in the hippocampus of neonatal rats.Methods A total of 112 male newborn Sprague Dawley(SD)rats aging 7 d were randomly divided into 8 groups(n=14):control group(Con1 group,intraperitoneally given normal saline for 5 d),and P50,P100 and P150 groups which received 50,100 mg/kg and 150 mg/kg propofol once per day for 5 d,respectively;and control 2 group(Con2 group,intraperitoneallyl receiving normal saline for 5 d,and appendectomy was performed under propofol anesthesia for the last day)and SP50,SP100 and SP150 groups which received 50,100 mg/kg and 150 mg/kg propofol for 5 d respectively,and appendectomy was performed under propofol anesthesia for the last day.Two months after the experiments,the level of Bcl-2(inhibitor of apoptosis proteins)and Bax(the proapoptotic protein)were detected using immunohistochemical methods.Results Compared with the Con1 group,the levels of Bax in the P50,P100 and P150 groups increased,while the amounts of Bcl-2 gradually decreased in the P100 and P150 groups(P<0.05).Compared with the P50 group,the levels of Bax increased(P<0.05),while the amounts of Bcl-2 decreased in the P100 and P150 groups.Compared with the Con2 group,the level of Bax in the SP50,SP100 and SP150 groups gradually increased(P<0.05),while the amount of Bcl-2 in the SP50 and SP150 groups gradually decreased(P<0.05).Compared with the SP50 group,the level of Bax in the SP100 and SP150 groups increased(P<0.05),while the amounts of Bcl-2 was decreased in the SP150 group(P<0.05).Conclusions Repeated administration of propofol can inhibit the activation of Bcl-2 and enhance the expression of Bax.It can promote the apoptosis of hippocampal neurons of newborn rats in a dose-dependent manor.
作者
韩丹
方浩
王颖林
金建华
Han Dan;Fang Hao;Wang Yinglin;Jin Jianhua(Department of Anesthesiology,East Hospital,Tongji University,Shanghai 200123,China;Department of Anesthesiology,Zhongshan Hospital,Fudan University,Shanghai 200032,China;Department of Anesthesiology,Jinshan Hospital,Fudan University,Shanghai 201508,China)
出处
《国际麻醉学与复苏杂志》
CAS
2019年第9期808-812,共5页
International Journal of Anesthesiology and Resuscitation
基金
上海市浦东新区卫生系统重要薄弱学科建设项目(新区卫计委PWZbr2017-18)。
