摘要
GP4蛋白是猪繁殖与呼吸综合征病毒(Porcine reproductive and respiratory syndrome virus,PRRSV)的次要结构蛋白,在病毒的复制与侵染中起重要作用。通过合成GP4蛋白的重叠多肽,采用间接ELISA、dot-ELISA的方法对GP4蛋白的B细胞表位进行初步筛选,得到1个免疫显性肽段Ⅰ(50)SCLRHGDSSSPTIRKSS(67),并对其抗体水平进行测定,为PRRSV的抗体检测及其新型表位疫苗的研究奠定基础。
GP4 protein is a minor structural protein of Porcine Reproductive and Respiratory Syndrome Virus(PRRSV),but it plays an important role in virus replication and infection.In this study,the overlapping peptides of GP4 protein were synthesized.The B cell epitope of GP4 protein was screened by indirect ELISA and dot-ELISA to obtain an immunodominant peptideⅠ(50)SCLRHGDSSSPTIRKSS(67),and its antibody level was measured,which helped the detection of PRRSV and its novel epitope vaccine.
作者
王爱萍
陈冰
刘红亮
周景明
陈玉梅
刘燕凯
祁艳华
张改平
WANG Aiping;CHEN Bing;LIU Hongliang;ZHOU Jingming;CHEN Yumei;LIU Yankai;QI Yanhua;ZHANG Gaiping(School of Life Sciences,Zhengzhou University,Zhengzhou 450000,China)
出处
《西北农业学报》
CAS
CSCD
北大核心
2019年第10期1561-1566,共6页
Acta Agriculturae Boreali-occidentalis Sinica
基金
国家自然科学基金(31472177)
智汇郑州1125计划~~
关键词
猪繁殖与呼吸综合征病毒
GP4蛋白
重叠多肽
B细胞表位
Porcine reproductive and respiratory syndrome virus
GP4 protein
Overlapping polypeptide
B cell epitope
